LOCUS EL595274 623 bp mRNA linear EST 07-MAR-2007
DEFINITION EoEST0304 Oil palm mesocarp-tissue cDNA Entry Library Elaeis
oleifera cDNA clone Mo17-220 5' similar to Unknown protein [Note:
similar to Oryza sativa (japonica cultivar-group) clone
Os11g0242400, genbank accession No: AP008217], mRNA sequence.
ACCESSION EL595274
VERSION EL595274.1
DBLINK BioSample: SAMN00152624
KEYWORDS EST.
SOURCE Elaeis oleifera (American oil palm)
ORGANISM Elaeis oleifera
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliopsida; Liliopsida; Arecaceae; Arecoideae;
Cocoseae; Elaeidinae; Elaeis.
REFERENCE 1 (bases 1 to 623)
AUTHORS Bhore,S.J., Amelia,K., Nurhuda,J. and Shah,F.H.
TITLE Analysis of expressed sequence tags (ESTs) generated from Elaeis
oleifera mesocarp tissue cDNA library (2007b)
JOURNAL Unpublished
COMMENT Contact: Bhore S.J.
Molecular Biology
Melaka Institute of Biotechnology
Lot 7, MITC City, Ayer Keroh, 75450, Melaka, Malaysia
Tel: 006 6 231 3532
Fax: 006 6 231 3276
Email: subhashbhore@yahoo.com
Insert length is not determined by restriction enzyme (BsrGI)
analysis. Only kanamycin resistant E. coli were selected,
cultivated, and ESTs were generated. Elaeis oleifera, seventeen
weeks old [Weeks After Anthesis (WAA)] mesocarp tissue cDNA library
was constructed using the CloneMiner cDNA library construction kit.
The cDNA carrying entry clones (pDNAs) were used in transformation
of E. coli and kanamycin resistant E. coli were selected,
cultivated, and ESTs were generated. After trimming and processing,
analysis of nucleotide sequences of ESTs has been completed, and
submitted in dbEST.
Seq primer: Universal primer M13 (Forward); 5' GTAAAACGACGGCCAG 3'
POLYA=No.
FEATURES Location/Qualifiers
source 1..623
/organism="Elaeis oleifera"
/mol_type="mRNA"
/cultivar="oleifera (Malaysian)"
/db_xref="taxon:80265"
/clone="Mo17-220"
/cell_type="Somatic"
/tissue_type="Mesocarp"
/clone_lib="SAMN00152624 Oil palm mesocarp-tissue cDNA
Entry Library"
/dev_stage="17 week after anthesis (WAA)"
/note="Organ: Fruit; Vector: Donor Vector, pDONR 222;
Site_1: BsrGI; Site_2: BsrGI; The cDNA library was
prepared following all the guidelines and the protocols
provided by manufacturer (Invitrogen Corporation) of the
CloneMiner cDNA Library Construction Kit. This CloneMiner
cDNA Library Construction Kit was used because it enables
us to construct highly representative cDNA libraries
without the risk of restriction enzymes cutting our
precious cDNA clones. Therefore, we can isolate fully
intact clones from high-quality cDNA library."
BASE COUNT 111 a 115 c 148 g 148 t
ORIGIN
1 gnacngngtt gggnnccagg accgggngna ccgnntggng gtttncgacc acgcaccgnn
61 tggaccgggt ggcgagtttc ngacnagtca ccgggtggac cgggtggcgg nttcggaccn
121 gggtttggtg gaccgtgcgg ntgcttatcg cctgtttgta ctccttgngt tgctgctggt
181 tgcnncagga tngttgnggc ccntttttcn gtgngcctcc acnctgacca agtctgcnga
241 ancngtngga taagatnaca tnatatgatn attntagcan anggacatnc anacttnttn
301 ncaanaanaa nngnncatng nnagnatcgg atnctattgt atgctcntgg gctagcacct
361 gnttttatnt tgnataggct gggaaattat ccattnggca nagactatct tgcttctctg
421 gtacaaagat ganncctttt ntntngtgna nnggtcccct nnngagnngg cntactttna
481 aagtnttttg gaatgcaaag ctncactctg atggnnntat nnnttgtctt cngncngnna
541 gctnatttcn tcttcganng ggnacaataa agggaanggt cccntncnac ccagttcnaa
601 nngncctaac aaaagctntg aag
//