LOCUS EB390646 585 bp mRNA linear EST 11-APR-2006 DEFINITION NPAC-aaa07c09.g2 N.brasiliensis_EST_L3N_SL1 Nippostrongylus brasiliensis cDNA similar to ref|NP_510484.1| Putative N-myristoylated membrane protein, with a transmembrane domain, of bilaterial origin (17.6 kD) [Caenorhabditis elegans] pir|T21142 hypothetical protein F20D1.1 - Caenorhabditis elegans emb|CAB01743.1| Hypothetical protein, mRNA sequence. ACCESSION EB390646 VERSION EB390646.1 DBLINK BioSample: SAMN00150071 KEYWORDS EST. SOURCE Nippostrongylus brasiliensis ORGANISM Nippostrongylus brasiliensis Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Strongyloidea; Heligmosomidae; Nippostrongylus. REFERENCE 1 (bases 1 to 585) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Makedonka Dautova Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Murray E. Selkirk, Imperial College London, m.selkirk@imperial.ac.uk Library constructed by Irina Ronko and Dr. Makedonka Mitreva Library sequenced by Washington University Genome Sequencing Center Contact Dr. Makedonka Mitreva (mmitreva@watson.wustl.edu) for information about this clone. original QR value of 853 was extended to value 854 (,) This trace has been recalled with phred original value before phred recall for SL was 18 original value before phred recall for SR was 861. FEATURES Location/Qualifiers source 1..585 /organism="Nippostrongylus brasiliensis" /mol_type="mRNA" /db_xref="taxon:27835" /clone_lib="SAMN00150071 N.brasiliensis_EST_L3N_SL1" /dev_stage="L3" /lab_host="GC10 cells" /note="Vector: pCRII-TOPO; Site_1: EcoRI; Site_2: EcoRI; The library was constructed by Irina Ronko and Dr. Makedonka Mitreva at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. Nippostrongylus brasiliensis L3 cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. RNA was provided by Dr. Murray E. Selkirk (m.selkirk@imperial.ac.uk) of Imperial College London, UK." BASE COUNT 129 a 134 c 135 g 187 t ORIGIN 1 tccctttgtg ggtgcgtttt ctcgccaaag gtgttggaat tctcggagga ttcctggcaa 61 ttttcttcgg cgctctcgga atactcacag taacgccttc ttgtctcgca gccgtattac 121 tacaaatggt gtccggactt ctaatcatcg cattggaggc cccgttttgt tgtatgttcg 181 tagactttat cgagaagatt gcccagttca gtgaatcgcg aaagcactgg catagagccg 241 cactctatgg aacaatgggc atagtgccac ttgttatttg cttcgaatta aacacattcc 301 tcggttccgg cactatattc gcatgtggga ctatttacgg tttcatggct ctgggaaaga 361 aggccgatcg aggtaccatg atggctgctg gagatcctgc atggagtccc caagtaaatc 421 agccttcaat gtcataggtg aatgaggctg ctatagatta tttccctctt ccgtgttctc 481 ctcttactcc gagctccaag ctttctattt attgatgttt ataaatacag tcttatacag 541 gtgttcatca ttagtttcaa aatgcggtgt ggtggtcact catct //