LOCUS       EB390646                 585 bp    mRNA    linear   EST 11-APR-2006
DEFINITION  NPAC-aaa07c09.g2 N.brasiliensis_EST_L3N_SL1 Nippostrongylus
            brasiliensis cDNA similar to ref|NP_510484.1| Putative
            N-myristoylated membrane protein, with a transmembrane domain, of
            bilaterial origin (17.6 kD) [Caenorhabditis elegans] pir|T21142
            hypothetical protein F20D1.1 - Caenorhabditis elegans
            emb|CAB01743.1| Hypothetical protein, mRNA sequence.
ACCESSION   EB390646
VERSION     EB390646.1
DBLINK      BioSample: SAMN00150071
KEYWORDS    EST.
SOURCE      Nippostrongylus brasiliensis
  ORGANISM  Nippostrongylus brasiliensis
            Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Strongylida;
            Trichostrongyloidea; Heligmonellidae; Nippostrongylinae;
            Nippostrongylus.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Makedonka Dautova Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material provided by Murray E. Selkirk, Imperial College
            London, m.selkirk@imperial.ac.uk Library constructed by Irina Ronko
            and Dr. Makedonka Mitreva Library sequenced by Washington
            University Genome Sequencing Center Contact Dr. Makedonka Mitreva
            (mmitreva@watson.wustl.edu) for information about this clone.
            original QR value of 853 was extended to value 854 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 18
            original value before phred recall for SR was 861.
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Nippostrongylus brasiliensis"
                     /mol_type="mRNA"
                     /db_xref="taxon:27835"
                     /clone_lib="SAMN00150071 N.brasiliensis_EST_L3N_SL1"
                     /dev_stage="L3"
                     /lab_host="GC10 cells"
                     /note="Vector: pCRII-TOPO; Site_1: EcoRI; Site_2: EcoRI;
                     The library was constructed by Irina Ronko and Dr.
                     Makedonka Mitreva at Washington University, St. Louis.
                     Oligo(dT)-SL1 PCR based library. Nippostrongylus
                     brasiliensis L3 cDNA PCR products of size >400 nucleotides
                     containing SL1 on the 5' end and oligo(dT) on the 3' end
                     were non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. RNA was provided
                     by Dr. Murray E. Selkirk (m.selkirk@imperial.ac.uk) of
                     Imperial College London, UK."
BASE COUNT          129 a          134 c          135 g          187 t
ORIGIN      
        1 tccctttgtg ggtgcgtttt ctcgccaaag gtgttggaat tctcggagga ttcctggcaa
       61 ttttcttcgg cgctctcgga atactcacag taacgccttc ttgtctcgca gccgtattac
      121 tacaaatggt gtccggactt ctaatcatcg cattggaggc cccgttttgt tgtatgttcg
      181 tagactttat cgagaagatt gcccagttca gtgaatcgcg aaagcactgg catagagccg
      241 cactctatgg aacaatgggc atagtgccac ttgttatttg cttcgaatta aacacattcc
      301 tcggttccgg cactatattc gcatgtggga ctatttacgg tttcatggct ctgggaaaga
      361 aggccgatcg aggtaccatg atggctgctg gagatcctgc atggagtccc caagtaaatc
      421 agccttcaat gtcataggtg aatgaggctg ctatagatta tttccctctt ccgtgttctc
      481 ctcttactcc gagctccaag ctttctattt attgatgttt ataaatacag tcttatacag
      541 gtgttcatca ttagtttcaa aatgcggtgt ggtggtcact catct
//