LOCUS EB186336 606 bp mRNA linear EST 05-APR-2006 DEFINITION NPAC-aaa07c09.b1 N.brasiliensis_EST_L3N_SL1 Nippostrongylus brasiliensis cDNA similar to ref|NP_510484.1| Putative N-myristoylated membrane protein, with a transmembrane domain, of bilaterial origin (17.6 kD) [Caenorhabditis elegans] pir|T21142 hypothetical protein F20D1.1 - Caenorhabditis elegans emb|CAB01743.1| Hypothetical protein, mRNA sequence. ACCESSION EB186336 VERSION EB186336.1 DBLINK BioSample: SAMN00150071 KEYWORDS EST. SOURCE Nippostrongylus brasiliensis ORGANISM Nippostrongylus brasiliensis Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Strongyloidea; Heligmosomidae; Nippostrongylus. REFERENCE 1 (bases 1 to 606) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Makedonka Dautova Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Murray E. Selkirk, Imperial College London, m.selkirk@imperial.ac.uk Library constructed by Irina Ronko and Dr. Makedonka Mitreva Library sequenced by Washington University Genome Sequencing Center Contact Dr. Makedonka Mitreva (mmitreva@watson.wustl.edu) for information about this clone. This trace has been recalled with phred original value before phred recall for SL was 75 original value before phred recall for SR was 824. FEATURES Location/Qualifiers source 1..606 /organism="Nippostrongylus brasiliensis" /mol_type="mRNA" /db_xref="taxon:27835" /clone_lib="SAMN00150071 N.brasiliensis_EST_L3N_SL1" /dev_stage="L3" /lab_host="GC10 cells" /note="Vector: pCRII-TOPO; Site_1: EcoRI; Site_2: EcoRI; The library was constructed by Irina Ronko and Dr. Makedonka Mitreva at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. Nippostrongylus brasiliensis L3 cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. RNA was provided by Dr. Murray E. Selkirk (m.selkirk@imperial.ac.uk) of Imperial College London, UK." BASE COUNT 134 a 145 c 150 g 177 t ORIGIN 1 ggcccttggg tttaattacc caagtttgag gtgacctatg agagcggcgg cgtcgggtat 61 ttccacacaa atgcaagcgg agcatgatcc aaatgcgaat ttcccgtggt tggtgcgttt 121 tctcgccaaa ggtgttggaa ttctcggagg attcctggca attttcttcg gcgctctcgg 181 aatactcaca gtaacgcctt cttgtctcgc agccgtatta ctacaaatgg tgtccggact 241 tctaatcatc gcattggagg ccccgttttg ttgtatgttc gtagacttta tcgagaagat 301 tgcccagttc agtgaatcgc gaaagcactg gcatagagcc gcactctatg gaacaatggg 361 catagtgcca cttgttattt gcttcgaatt aaacacattc ctcggttccg gcactatatt 421 cgcatgtggg actatttacg gtttcatggc tctgggaaag aaggccgatc gaggtaccat 481 gatggctgct ggagatcctg catggagtcc ccaagtaaat cagccttcaa tgtcataggt 541 gaatgaggct gctatagatt atttccctct tccgtgttct cctcttactc cgagctccaa 601 gctttc //