LOCUS EB182931 551 bp mRNA linear EST 05-APR-2006
DEFINITION AHAH-aaa12h02.g1 Pristionchus_pacificus_L4_pDNR_LIB Pristionchus
pacificus cDNA 5' similar to ref|NP_492721.1| Putative membrane
protein, with at least 2 transmembrane domains, of eukaryotic
origin (28.5 kD) [Caenorhabditis elegans] pir|T21337 hypothetical
protein F25D7.1 - Caenorhabditis elegans emb|CAB01696.1|
Hypothetical protein F25D7, mRNA sequence.
ACCESSION EB182931
VERSION EB182931.1
DBLINK BioSample: SAMN00149987
KEYWORDS EST.
SOURCE Pristionchus pacificus
ORGANISM Pristionchus pacificus
Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
Rhabditina; Diplogasteromorpha; Diplogasteroidea;
Neodiplogasteridae; Pristionchus.
REFERENCE 1 (bases 1 to 551)
AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
Scott,A.L., McCarter,J.P. and Wilson,R.
TITLE WashU Nematode EST Project
JOURNAL Unpublished
COMMENT Contact: Makedonka Dautova Mitreva
WashU Nematode EST Project
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
Tel: 314 286 1800
Fax: 314 286 1810
Email: submissions@watson.wustl.edu
Library material provided by Ralf Sommer,Max-Planck
Institute,Tubingen,Germany Library constructed by Irina Ronko and
Dr. Makedonka Mitreva Library sequenced by Washington University
Genome Sequencing Center Contact Dr. Makedonka Mitreva
(mmitreva@watson.wustl.edu) for information about this clone.
This trace has been recalled with phred
original value before phred recall for SL was 153
original value before phred recall for SR was 787
Seq primer: -21UPpOT.
FEATURES Location/Qualifiers
source 1..551
/organism="Pristionchus pacificus"
/mol_type="mRNA"
/strain="Californian strain PS312"
/db_xref="taxon:54126"
/clone_lib="SAMN00149987
Pristionchus_pacificus_L4_pDNR_LIB"
/dev_stage="L4"
/lab_host="GC10 cells"
/note="Vector: pDNR-LIB; Site_1: SfiI B; Site_2: SfiI A;
The library was created by Irina Ronko and Dr. Makedonka
Mitreva (mmitreva@watson.wustl.edu) at Washington
University, St. Louis using Creator SMART cDNA library
construction kit from BD Biosciences. The RNA was provided
by Dr. Ralf J. Sommer of Max-Planck Institute, Tuebingen,
Germany"
BASE COUNT 121 a 135 c 146 g 149 t
ORIGIN
1 ggccgggtcc tctacaatta ctcgaaaagc gtggaacaag gagtcttcga tggaaggcct
61 gctgactacc ttttcatgct catattcagt ggaattgttt gctcgggcct ctgctttgct
121 gcaagcattc ccttcatgtt ggagccaatg gtcatggctg ttctttatgt ctggagtcag
181 ctgaataagg acactattgt ctccttctgg ttcggaacca gattcaaggc gatgtacctt
241 ccctggattc ttgttggatt caacgcaatt cttcgaggcg gtggaatcaa cgagcttttg
301 ggaattctca ttggccacac ttacttcttc ctggcattca agtaccctca ggaccacggt
361 ggcgaatcgt tgctccgtac tcccgagttc ctatatcgat tccttccctc cgagcaaggc
421 ggagttcact ccttcggggg atctactgtc gagcagagaa gagctgctgg agcaggcggc
481 aacggctatg ccaggggaag aggacaacaa ctcggagcgg ctagggatct tgatgagtag
541 atatgtacaa a
//