LOCUS DY527233 751 bp mRNA linear EST 22-FEB-2006
DEFINITION BAAC-PNP1299B03.g2 C.remanei EST SB146 Caenorhabditis remanei cDNA
5' similar to ref|NP_492721.1| Putative membrane protein, with at
least 2 transmembrane domains, of eukaryotic origin (28.5 kD)
[Caenorhabditis elegans] pir||T21337 hypothetical protein F25D7.1 -
Caenorhabditis elegans emb|CAB01696.1| Hypothetical protein F25D7,
mRNA sequence.
ACCESSION DY527233
VERSION DY527233.1
DBLINK BioSample: SAMN00176426
KEYWORDS EST.
SOURCE Caenorhabditis remanei
ORGANISM Caenorhabditis remanei
Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
Caenorhabditis.
REFERENCE 1 (bases 1 to 751)
AUTHORS Mitreva,M., Fulton,L., Nash,W., Ronko,I., Theising,B., Martin,J.,
Rudel,D., Haag,E., Spieth,J. and Wilson,R.
TITLE WashU Caenorhabditis remanei EST Project
JOURNAL Unpublished
COMMENT Contact: Makedonka Dautova Mitreva
WashU Nematode EST Project
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
Tel: 314 286 1800
Fax: 314 286 1810
Email: submissions@watson.wustl.edu
Librarymaterial provided by J.Kimble Lab, University of Wisc. And
HHMI Libraryconstructed by David Rudel and Eric Haag
Librarysequenced by Washington University Genome Sequencing Center
Thistrace has been recalled with phred
originalvalue before phred recall for SL was 0
originalvalue before phred recall for SR was 847
Seq primer: T7True.
FEATURES Location/Qualifiers
source 1..751
/organism="Caenorhabditis remanei"
/mol_type="mRNA"
/strain="SB146"
/db_xref="taxon:31234"
/sex="male-female, 50-50"
/clone_lib="SAMN00176426 C.remanei EST SB146"
/dev_stage="Mixed"
/lab_host="DH10B (ZIP)"
/note="Vector: GIBCO/BRL/Life Technologies lambda zip/lox,
converted to pZL1; Site_1: Not I; Site_2: Sal I;
Approximately 1.2 million independent transformants were
amplified. Size selection during construction mainly
excluded very small (i.e. less than 400nt) mRNA. The
entire library was excised in pZL1 from Lambda ZipLox
Phage. Y1090 (ZL) used for plaque plating and DH10B (ZIP)
used to convert the phage into plasmids."
BASE COUNT 177 a 175 c 185 g 214 t
ORIGIN
1 cggtccggat tcccgggtcg acccacgcgt ccgcggacgc gtgggcggac gcgcctttgg
61 atttagaaag cttccttttg ggaataccag tcgttactag gtactggttt ctcgcgtcga
121 cgatcgtacc tcttctcggt agattcggat tcatccagat gcaatggatg taccttgaat
181 gggatctagt cgtcagcaag ttccatatat ggagacctct tacatctgca atatactacc
241 cgatcactcc acagactgga ttccactggc taatgatgtg ctattttcta tacaactact
301 caaaagcact agaaagagaa acgtatcgtg gcagatcagc tgactacctt ttcatgctca
361 tattcaactg gttcttctgt gtgggaatct gtatggcgat gggcatttac ttcttactcg
421 aaccaatggt gatgtcagtt ctttacgttt ggtgccaagt caacaaagac acgatcgtat
481 ccttctggtt cggaatgagg ttcccagctc gttatctacc atgggtactc tggggcttca
541 acgctgttct tcgtggtggt ggaacaaacg aactcgttgg aatcatcgta ggacacgcct
601 acttcttcgt ggcactcaag tatccagatg aatatggtgt tgatctgata tctactccgg
661 aatttttgca tcgtttgatt ccggatgaag acggaggtat tcatggacaa gacggtgata
721 tcagaggggc cagacaacaa ccacgtggtg g
//