LOCUS DY525760 776 bp mRNA linear EST 22-FEB-2006 DEFINITION BAAC-PNP1305B14.g1 C.remanei EST SB146 Caenorhabditis remanei cDNA 5' similar to ref|NP_510484.1| Putative N-myristoylated membrane protein, with a transmembrane domain, of bilaterial origin (17.6 kD) [Caenorhabditis elegans] pir||T21142 hypothetical protein F20D1.1 - Caenorhabditis elegans emb|CAB01743.1| Hypothetical protein, mRNA sequence. ACCESSION DY525760 VERSION DY525760.1 DBLINK BioSample: SAMN00176426 KEYWORDS EST. SOURCE Caenorhabditis remanei ORGANISM Caenorhabditis remanei Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis. REFERENCE 1 (bases 1 to 776) AUTHORS Mitreva,M., Fulton,L., Nash,W., Ronko,I., Theising,B., Martin,J., Rudel,D., Haag,E., Spieth,J. and Wilson,R. TITLE WashU Caenorhabditis remanei EST Project JOURNAL Unpublished COMMENT Contact: Makedonka Dautova Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Librarymaterial provided by J.Kimble Lab, University of Wisc. And HHMI Libraryconstructed by David Rudel and Eric Haag Librarysequenced by Washington University Genome Sequencing Center Thistrace has been recalled with phred originalvalue before phred recall for SL was 0 originalvalue before phred recall for SR was 798 Seq primer: T7True. FEATURES Location/Qualifiers source 1..776 /organism="Caenorhabditis remanei" /mol_type="mRNA" /strain="SB146" /db_xref="taxon:31234" /sex="male-female, 50-50" /clone_lib="SAMN00176426 C.remanei EST SB146" /dev_stage="Mixed" /lab_host="DH10B (ZIP)" /note="Vector: GIBCO/BRL/Life Technologies lambda zip/lox, converted to pZL1; Site_1: Not I; Site_2: Sal I; Approximately 1.2 million independent transformants were amplified. Size selection during construction mainly excluded very small (i.e. less than 400nt) mRNA. The entire library was excised in pZL1 from Lambda ZipLox Phage. Y1090 (ZL) used for plaque plating and DH10B (ZIP) used to convert the phage into plasmids." BASE COUNT 154 a 234 c 154 g 233 t ORIGIN 1 taccggtccg gattcccggg tcgacccacg cgtccgggta attgttctct ttcgacacgg 61 tggctgccgc atctggagtt agcacgcaaa tgcaagcgga gcacgatccg aacgccgcgt 121 ttccatggtg ggttcgcttc ttggccaaag gagtcgccat cctcggagga ttcctctcgc 181 tcttctttgg agttctcgga ctcatcactc tctcggcaac atgtatggtc gccattcttc 241 tccaaatgac atccggagca ctcgtcatcg ccttggaagc tccgttctgc tgccagttcg 301 tcgacttcat tgagaagatt gcccgtttca gcgagtctcg tcaactctgg cacaaggctg 361 ctatctacgg agtcatgggc ctcatcccaa tcttcctctg catcgaactc aacaccatcc 421 ttggcagtgg aaccatcttt gcgtctggtg tcatctacgg attcatggct ctcggaaaga 481 aggccgatcg caacaatatg atggctgctg gagatccggc atggagtcca caagtcaacc 541 agtccaacat cccataaacc atatactcct gaatatgaaa gtacctccta tccagtaatt 601 tttcactttg tctgtctttt ctctcttcct tccctcaatt cccatattta tgtctcgtat 661 ttattttcct tactttttct tccatctgct ttactccctc cccccctttc tcactncagc 721 gttttatctc ccgttcatgc aattttgaca caaccaacta gtttggattc gacttt //