LOCUS       DY525760                 776 bp    mRNA    linear   EST 22-FEB-2006
DEFINITION  BAAC-PNP1305B14.g1 C.remanei EST SB146 Caenorhabditis remanei cDNA
            5' similar to ref|NP_510484.1| Putative N-myristoylated membrane
            protein, with a transmembrane domain, of bilaterial origin (17.6
            kD) [Caenorhabditis elegans] pir||T21142 hypothetical protein
            F20D1.1 - Caenorhabditis elegans emb|CAB01743.1| Hypothetical
            protein, mRNA sequence.
ACCESSION   DY525760
VERSION     DY525760.1
DBLINK      BioSample: SAMN00176426
KEYWORDS    EST.
SOURCE      Caenorhabditis remanei
  ORGANISM  Caenorhabditis remanei
            Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
            Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
            Caenorhabditis.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Mitreva,M., Fulton,L., Nash,W., Ronko,I., Theising,B., Martin,J.,
            Rudel,D., Haag,E., Spieth,J. and Wilson,R.
  TITLE     WashU Caenorhabditis remanei EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Makedonka Dautova Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Librarymaterial provided by J.Kimble Lab, University of Wisc. And
            HHMI Libraryconstructed by David Rudel and Eric Haag
            Librarysequenced by Washington University Genome Sequencing Center
            Thistrace has been recalled with phred
            originalvalue before phred recall for SL was 0
            originalvalue before phred recall for SR was 798
            Seq primer: T7True.
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Caenorhabditis remanei"
                     /mol_type="mRNA"
                     /strain="SB146"
                     /db_xref="taxon:31234"
                     /sex="male-female, 50-50"
                     /clone_lib="SAMN00176426 C.remanei EST SB146"
                     /dev_stage="Mixed"
                     /lab_host="DH10B (ZIP)"
                     /note="Vector: GIBCO/BRL/Life Technologies lambda zip/lox,
                     converted to pZL1; Site_1: Not I; Site_2: Sal I;
                     Approximately 1.2 million independent transformants were
                     amplified. Size selection during construction mainly
                     excluded very small (i.e. less than 400nt) mRNA. The
                     entire library was excised in pZL1 from Lambda ZipLox
                     Phage. Y1090 (ZL) used for plaque plating and DH10B (ZIP)
                     used to convert the phage into plasmids."
BASE COUNT          154 a          234 c          154 g          233 t
ORIGIN      
        1 taccggtccg gattcccggg tcgacccacg cgtccgggta attgttctct ttcgacacgg
       61 tggctgccgc atctggagtt agcacgcaaa tgcaagcgga gcacgatccg aacgccgcgt
      121 ttccatggtg ggttcgcttc ttggccaaag gagtcgccat cctcggagga ttcctctcgc
      181 tcttctttgg agttctcgga ctcatcactc tctcggcaac atgtatggtc gccattcttc
      241 tccaaatgac atccggagca ctcgtcatcg ccttggaagc tccgttctgc tgccagttcg
      301 tcgacttcat tgagaagatt gcccgtttca gcgagtctcg tcaactctgg cacaaggctg
      361 ctatctacgg agtcatgggc ctcatcccaa tcttcctctg catcgaactc aacaccatcc
      421 ttggcagtgg aaccatcttt gcgtctggtg tcatctacgg attcatggct ctcggaaaga
      481 aggccgatcg caacaatatg atggctgctg gagatccggc atggagtcca caagtcaacc
      541 agtccaacat cccataaacc atatactcct gaatatgaaa gtacctccta tccagtaatt
      601 tttcactttg tctgtctttt ctctcttcct tccctcaatt cccatattta tgtctcgtat
      661 ttattttcct tactttttct tccatctgct ttactccctc cccccctttc tcactncagc
      721 gttttatctc ccgttcatgc aattttgaca caaccaacta gtttggattc gacttt
//