LOCUS DT042616 737 bp mRNA linear EST 06-OCT-2005 DEFINITION Mdrtp1010C08.g1 Apple_EST_Mdrtp Malus domestica x Malus sieversii cDNA similar to pir|F86447 F5D14.6 protein - Arabidopsis thaliana gb|AAF81326.1|AC007767_6 Strong similarity to an unknown protein F19D11.4 gi|7485759 from Arabidopsis thaliana BAC F19D11 gb|AC005310. EST gb|AV535485 comes from this gene, mRNA sequence. ACCESSION DT042616 VERSION DT042616.1 DBLINK BioSample: SAMN00176552 KEYWORDS EST. SOURCE Malus domestica x Malus sieversii ORGANISM Malus domestica x Malus sieversii Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Rosales; Rosaceae; Amygdaloideae; Maleae; Malus. REFERENCE 1 (bases 1 to 737) AUTHORS Korban,S., Vodkin,L., Liu,L., Gasic,K., Gonzales,O., Hernandez,A., Aldwinckle,H., Malnoy,M., Carroll,N., Goldsbrough,P., Orvis,K., Clifton,S., Pape,D., Marra,M., Hillier,L., Martin,J., Wylie,T., Dante,M., Theising,B., Bowers,Y., Gibbons,M., Ritter,E., Ronko,I., Tsagareishvili,R., Kennedy,S., Waterston,R. and Wilson,R. TITLE Apple Functional Genomics grant - NSF 0321702 JOURNAL Unpublished COMMENT Contact: Schuyler S. Korban Apple Functional Genomics grant - NSF 0321702 Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by S.Korban Library constructed by K. Gasic Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 90 original value before phred recall for SR was 906. FEATURES Location/Qualifiers source 1..737 /organism="Malus domestica x Malus sieversii" /mol_type="mRNA" /strain="Geneva 3041 x Malus sieversii" /db_xref="taxon:339852" /tissue_type="Root" /clone_lib="SAMN00176552 Apple_EST_Mdrtp" /dev_stage="phytophtora challenged young root" /lab_host="DH10B ampicillin resistant" /note="Vector: pBluescript II SK (+); Site_1: Not I; Site_2: EcoRI; Geneva 3041 x Malus siversii apple rootstock. Total RNA was extracted separately from whole root using the 'pine tree' method. Poly(A)+mRNA was isolated twice from total RNA from each stage using the Oligotex Direct mRNA kit (Qiagen). mRNA was reverse transcribed into double stranded cDNA using a modified oligo18(dT) primer. Double stranded cDNAs were size selected (more than 450 bp), adaptored with EcoRI adapters at both ends and then digested with NotI. The cDNAs were then directionally cloned into EcoR1-NotI digested pBS II SK(+) phagemid vector(Stratagene). The total number of white colony forming units (cfu) was 1.5 x 10^6 cfu cfu (colony forming units). The background of empty clones was less than 6%. Inserts ranged from 0.5kb to 4 kb, as determined by PCR." BASE COUNT 210 a 181 c 157 g 189 t ORIGIN 1 gaattccgtt gctgtcgctt ccttgtgcga cagtgagaat gagtaattcg aattcatcgc 61 cggcacattc gtcgatatcg acgacagcga tagccggaga aagagaacga agcaattcca 121 atcctgccgc cgccgccgct ctctccgacg acctcttctt cccctccgac ctcatttcca 181 ttcaagatcg caaacaagaa gcattacttg ttataaaatc cgatcttatg gatgcactta 241 acaaggtggt taaatccctt gaagaagata attggatgtt cgaagggccg cgccatcgcg 301 ttatccctat atcaagacaa ggtagtgaac acaacaaacc acttgccaga aaacacggca 361 tgccataaaa gaatgtgtag tcatagaagc ataactccaa ttcctgttct ctgatcttgc 421 tcccacaccc caccccttga tctgccaacc catctagcga ttatccctct aaccttggag 481 acggcagagg catggaggtg acaacctctg gacattgacg gcggttttct ccagaagcac 541 aaggaagcat caaataaatg gaatatggca ccaccaaaat gattttcagc caggaaggat 601 aatgcgttct tgcatatgta tgatgttaac tagcatgagg gctcctggat tgctgctttg 661 gtacgtgatt acaaacattt tgggttttct tctgtctgtt tggtaaatga gtcaatttat 721 gagaaggcaa agcaatt //