LOCUS       CZ482184                  32 bp    DNA     linear   GSS 16-FEB-2014
DEFINITION  e04423-5prime Exelixis piggyBac RB insertions Drosophila
            melanogaster genomic Sequence recovered from 5' end of piggyBac,
            genomic survey sequence.
ACCESSION   CZ482184
VERSION     CZ482184.1
DBLINK      BioSample: SAMN00183073
KEYWORDS    GSS.
SOURCE      Drosophila melanogaster (fruit fly)
  ORGANISM  Drosophila melanogaster
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Diptera; Brachycera;
            Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora.
REFERENCE   1  (bases 1 to 32)
  AUTHORS   Thibault,S.T., Singer,M.A., Miyazaki,W.Y., Milash,B., Dompe,N.A.,
            Singh,C.M., Buchholz,R., Demsky,M., Fawcett,R., Francis-Lang,H.L.,
            Ryner,L., Cheung,L.M., Chong,A., Erickson,C., Fisher,W.W.,
            Greer,K., Hartouni,S.R., Howie,E., Jakkula,L., Joo,D., Killpack,K.,
            Laufer,A., Mazzotta,J., Smith,R.D., Stevens,L.M., Stuber,C.,
            Tan,L.R., Ventura,R., Woo,A., Zakrajsek,I., Zhao,L., Chen,F.,
            Swimmer,C., Kopczynski,C., Duyk,G., Winberg,M.L. and Margolis,J.
  TITLE     A complementary transposon tool kit for Drosophila melanogaster
            using P and piggyBac
  JOURNAL   Nat. Genet. 36 (3), 283-287 (2004)
   PUBMED   14981521
COMMENT     Contact: Roger A Hoskins
            Life Sciences Division
            Lawrence Berkeley National Laboratory
            Mailstop 977-0164, One Cyclotron Road, Berkeley, CA 94720, USA
            Tel: 510 486 4015
            Fax: 510 486 6798
            Email: rhoskins@lbl.gov
            
            Sequence recovery method was inverse PCR.
            Sequence orientation is forward strand relative to 5' end of
            piggyBac element.
            The piggyBac insertion position is 29 in the 32 bases. This
            insertion position refers to the first base of the 4 base TTAA
            target recognition sequence.
            Class: transposon insertion site.
FEATURES             Location/Qualifiers
     source          1..32
                     /organism="Drosophila melanogaster"
                     /mol_type="genomic DNA"
                     /strain="isogenic w- strain"
                     /db_xref="BDGP_INS:e04423"
                     /db_xref="taxon:7227"
                     /clone_lib="SAMN00183073 Exelixis piggyBac RB insertions"
                     /note="Vector: piggyBac RB (GenBank accession number
                     AY515147); An isogenic w- Drosophila melanogaster strain
                     was mutagenized by remobilization of transposable
                     elements. We remobilized RB the element using the
                     constitutive alpha-1 tubulin:piggyBac transposase source.
                     We remobilized the RB element from a single X chromosome
                     insertion in dysgenic males. We outcrossed dysgenic males
                     in vials to the isogenic w- strain and selected new hops
                     in the following generation. All lines were mapped to a
                     chromosome by standard genetic methods, examined for
                     homozygous viability, and used for recovery of flanking
                     genomic sequence by inverse PCR."
BASE COUNT           14 a            2 c            5 g           11 t
ORIGIN      
        1 atatagtata atatgggcat agaatcattt aa
//