LOCUS       CZ482183                  65 bp    DNA     linear   GSS 16-FEB-2014
DEFINITION  e04423-3prime Exelixis piggyBac RB insertions Drosophila
            melanogaster genomic Sequence recovered from 3' end of piggyBac,
            genomic survey sequence.
ACCESSION   CZ482183
VERSION     CZ482183.1
DBLINK      BioSample: SAMN00183073
KEYWORDS    GSS.
SOURCE      Drosophila melanogaster (fruit fly)
  ORGANISM  Drosophila melanogaster
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Diptera; Brachycera;
            Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora.
REFERENCE   1  (bases 1 to 65)
  AUTHORS   Thibault,S.T., Singer,M.A., Miyazaki,W.Y., Milash,B., Dompe,N.A.,
            Singh,C.M., Buchholz,R., Demsky,M., Fawcett,R., Francis-Lang,H.L.,
            Ryner,L., Cheung,L.M., Chong,A., Erickson,C., Fisher,W.W.,
            Greer,K., Hartouni,S.R., Howie,E., Jakkula,L., Joo,D., Killpack,K.,
            Laufer,A., Mazzotta,J., Smith,R.D., Stevens,L.M., Stuber,C.,
            Tan,L.R., Ventura,R., Woo,A., Zakrajsek,I., Zhao,L., Chen,F.,
            Swimmer,C., Kopczynski,C., Duyk,G., Winberg,M.L. and Margolis,J.
  TITLE     A complementary transposon tool kit for Drosophila melanogaster
            using P and piggyBac
  JOURNAL   Nat. Genet. 36 (3), 283-287 (2004)
   PUBMED   14981521
COMMENT     Contact: Roger A Hoskins
            Life Sciences Division
            Lawrence Berkeley National Laboratory
            Mailstop 977-0164, One Cyclotron Road, Berkeley, CA 94720, USA
            Tel: 510 486 4015
            Fax: 510 486 6798
            Email: rhoskins@lbl.gov
            
            Sequence recovery method was inverse PCR.
            Sequence orientation is forward strand relative to 5' end of
            piggyBac element.
            The piggyBac insertion position is 1 in the 65 bases. This
            insertion position refers to the first base of the 4 base TTAA
            target recognition sequence.
            Class: transposon insertion site.
FEATURES             Location/Qualifiers
     source          1..65
                     /organism="Drosophila melanogaster"
                     /mol_type="genomic DNA"
                     /strain="isogenic w- strain"
                     /db_xref="BDGP_INS:e04423"
                     /db_xref="taxon:7227"
                     /clone_lib="SAMN00183073 Exelixis piggyBac RB insertions"
                     /note="Vector: piggyBac RB (GenBank accession number
                     AY515147); An isogenic w- Drosophila melanogaster strain
                     was mutagenized by remobilization of transposable
                     elements. We remobilized RB the element using the
                     constitutive alpha-1 tubulin:piggyBac transposase source.
                     We remobilized the RB element from a single X chromosome
                     insertion in dysgenic males. We outcrossed dysgenic males
                     in vials to the isogenic w- strain and selected new hops
                     in the following generation. All lines were mapped to a
                     chromosome by standard genetic methods, examined for
                     homozygous viability, and used for recovery of flanking
                     genomic sequence by inverse PCR."
BASE COUNT           21 a           12 c           15 g           17 t
ORIGIN      
        1 ttaaggagat tgcaacgacg cgaggttttc aaaatgatcg gtttctaaag aacacactta
       61 tgcgc
//