LOCUS CZ482183 65 bp DNA linear GSS 16-FEB-2014 DEFINITION e04423-3prime Exelixis piggyBac RB insertions Drosophila melanogaster genomic Sequence recovered from 3' end of piggyBac, genomic survey sequence. ACCESSION CZ482183 VERSION CZ482183.1 DBLINK BioSample: SAMN00183073 KEYWORDS GSS. SOURCE Drosophila melanogaster (fruit fly) ORGANISM Drosophila melanogaster Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora. REFERENCE 1 (bases 1 to 65) AUTHORS Thibault,S.T., Singer,M.A., Miyazaki,W.Y., Milash,B., Dompe,N.A., Singh,C.M., Buchholz,R., Demsky,M., Fawcett,R., Francis-Lang,H.L., Ryner,L., Cheung,L.M., Chong,A., Erickson,C., Fisher,W.W., Greer,K., Hartouni,S.R., Howie,E., Jakkula,L., Joo,D., Killpack,K., Laufer,A., Mazzotta,J., Smith,R.D., Stevens,L.M., Stuber,C., Tan,L.R., Ventura,R., Woo,A., Zakrajsek,I., Zhao,L., Chen,F., Swimmer,C., Kopczynski,C., Duyk,G., Winberg,M.L. and Margolis,J. TITLE A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac JOURNAL Nat. Genet. 36 (3), 283-287 (2004) PUBMED 14981521 COMMENT Contact: Roger A Hoskins Life Sciences Division Lawrence Berkeley National Laboratory Mailstop 977-0164, One Cyclotron Road, Berkeley, CA 94720, USA Tel: 510 486 4015 Fax: 510 486 6798 Email: rhoskins@lbl.gov Sequence recovery method was inverse PCR. Sequence orientation is forward strand relative to 5' end of piggyBac element. The piggyBac insertion position is 1 in the 65 bases. This insertion position refers to the first base of the 4 base TTAA target recognition sequence. Class: transposon insertion site. FEATURES Location/Qualifiers source 1..65 /organism="Drosophila melanogaster" /mol_type="genomic DNA" /strain="isogenic w- strain" /db_xref="BDGP_INS:e04423" /db_xref="taxon:7227" /clone_lib="SAMN00183073 Exelixis piggyBac RB insertions" /note="Vector: piggyBac RB (GenBank accession number AY515147); An isogenic w- Drosophila melanogaster strain was mutagenized by remobilization of transposable elements. We remobilized RB the element using the constitutive alpha-1 tubulin:piggyBac transposase source. We remobilized the RB element from a single X chromosome insertion in dysgenic males. We outcrossed dysgenic males in vials to the isogenic w- strain and selected new hops in the following generation. All lines were mapped to a chromosome by standard genetic methods, examined for homozygous viability, and used for recovery of flanking genomic sequence by inverse PCR." BASE COUNT 21 a 12 c 15 g 17 t ORIGIN 1 ttaaggagat tgcaacgacg cgaggttttc aaaatgatcg gtttctaaag aacacactta 61 tgcgc //