LOCUS CZ480286 158 bp DNA linear GSS 16-FEB-2014 DEFINITION e02507-5prime Exelixis piggyBac RB insertions Drosophila melanogaster genomic Sequence recovered from 5' end of piggyBac, genomic survey sequence. ACCESSION CZ480286 VERSION CZ480286.1 DBLINK BioSample: SAMN00183073 KEYWORDS GSS. SOURCE Drosophila melanogaster (fruit fly) ORGANISM Drosophila melanogaster Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora. REFERENCE 1 (bases 1 to 158) AUTHORS Thibault,S.T., Singer,M.A., Miyazaki,W.Y., Milash,B., Dompe,N.A., Singh,C.M., Buchholz,R., Demsky,M., Fawcett,R., Francis-Lang,H.L., Ryner,L., Cheung,L.M., Chong,A., Erickson,C., Fisher,W.W., Greer,K., Hartouni,S.R., Howie,E., Jakkula,L., Joo,D., Killpack,K., Laufer,A., Mazzotta,J., Smith,R.D., Stevens,L.M., Stuber,C., Tan,L.R., Ventura,R., Woo,A., Zakrajsek,I., Zhao,L., Chen,F., Swimmer,C., Kopczynski,C., Duyk,G., Winberg,M.L. and Margolis,J. TITLE A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac JOURNAL Nat. Genet. 36 (3), 283-287 (2004) PUBMED 14981521 COMMENT Contact: Roger A Hoskins Life Sciences Division Lawrence Berkeley National Laboratory Mailstop 977-0164, One Cyclotron Road, Berkeley, CA 94720, USA Tel: 510 486 4015 Fax: 510 486 6798 Email: rhoskins@lbl.gov Sequence recovery method was inverse PCR. Sequence orientation is forward strand relative to 5' end of piggyBac element. The piggyBac insertion position is 155 in the 158 bases. This insertion position refers to the first base of the 4 base TTAA target recognition sequence. Class: transposon insertion site. FEATURES Location/Qualifiers source 1..158 /organism="Drosophila melanogaster" /mol_type="genomic DNA" /strain="isogenic w- strain" /db_xref="BDGP_INS:e02507" /db_xref="taxon:7227" /clone_lib="SAMN00183073 Exelixis piggyBac RB insertions" /note="Vector: piggyBac RB (GenBank accession number AY515147); An isogenic w- Drosophila melanogaster strain was mutagenized by remobilization of transposable elements. We remobilized RB the element using the constitutive alpha-1 tubulin:piggyBac transposase source. We remobilized the RB element from a single X chromosome insertion in dysgenic males. We outcrossed dysgenic males in vials to the isogenic w- strain and selected new hops in the following generation. All lines were mapped to a chromosome by standard genetic methods, examined for homozygous viability, and used for recovery of flanking genomic sequence by inverse PCR." BASE COUNT 37 a 25 c 40 g 56 t ORIGIN 1 gcgatgacga gcttgttggt gaggattctg acagtgaaat atcgggcttt tttagttccc 61 gcgaattgcg ttgcagagct tgtagaattt tgtagatttg gggacccaac aataccggtt 121 ctattgtttg tgaaactaat tttcatctga ttcgttaa //