LOCUS CZ478937 390 bp DNA linear GSS 16-FEB-2014 DEFINITION e01120-3prime Exelixis piggyBac RB insertions Drosophila melanogaster genomic Sequence recovered from 3' end of piggyBac, genomic survey sequence. ACCESSION CZ478937 VERSION CZ478937.1 DBLINK BioSample: SAMN00183073 KEYWORDS GSS. SOURCE Drosophila melanogaster (fruit fly) ORGANISM Drosophila melanogaster Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora. REFERENCE 1 (bases 1 to 390) AUTHORS Thibault,S.T., Singer,M.A., Miyazaki,W.Y., Milash,B., Dompe,N.A., Singh,C.M., Buchholz,R., Demsky,M., Fawcett,R., Francis-Lang,H.L., Ryner,L., Cheung,L.M., Chong,A., Erickson,C., Fisher,W.W., Greer,K., Hartouni,S.R., Howie,E., Jakkula,L., Joo,D., Killpack,K., Laufer,A., Mazzotta,J., Smith,R.D., Stevens,L.M., Stuber,C., Tan,L.R., Ventura,R., Woo,A., Zakrajsek,I., Zhao,L., Chen,F., Swimmer,C., Kopczynski,C., Duyk,G., Winberg,M.L. and Margolis,J. TITLE A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac JOURNAL Nat. Genet. 36 (3), 283-287 (2004) PUBMED 14981521 COMMENT Contact: Roger A Hoskins Life Sciences Division Lawrence Berkeley National Laboratory Mailstop 977-0164, One Cyclotron Road, Berkeley, CA 94720, USA Tel: 510 486 4015 Fax: 510 486 6798 Email: rhoskins@lbl.gov Sequence recovery method was inverse PCR. Sequence orientation is forward strand relative to 5' end of piggyBac element. The piggyBac insertion position is 1 in the 390 bases. This insertion position refers to the first base of the 4 base TTAA target recognition sequence. Class: transposon insertion site. FEATURES Location/Qualifiers source 1..390 /organism="Drosophila melanogaster" /mol_type="genomic DNA" /strain="isogenic w- strain" /db_xref="BDGP_INS:e01120" /db_xref="taxon:7227" /clone_lib="SAMN00183073 Exelixis piggyBac RB insertions" /note="Vector: piggyBac RB (GenBank accession number AY515147); An isogenic w- Drosophila melanogaster strain was mutagenized by remobilization of transposable elements. We remobilized RB the element using the constitutive alpha-1 tubulin:piggyBac transposase source. We remobilized the RB element from a single X chromosome insertion in dysgenic males. We outcrossed dysgenic males in vials to the isogenic w- strain and selected new hops in the following generation. All lines were mapped to a chromosome by standard genetic methods, examined for homozygous viability, and used for recovery of flanking genomic sequence by inverse PCR." BASE COUNT 106 a 87 c 69 g 128 t ORIGIN 1 ttaagcatat aaaggcattt cgaattgttt tgttaactat aaatcaccat tgtcaagtaa 61 ttcttccaaa atgcttttct aattcacccc tcctgctctt aaacaataca gaagtgttct 121 agatatattt aaaactttat ttgtgatcat catgcgattc tcttagccgc tctcccaaac 181 tctctcgata tcgattttca tgtgccggct actttgaatt gagattgagc acgaagatat 241 tagcactctc tcaattgttt ttgcctctct gtgtgcgttt gcacttgatt tcttaataca 301 atgcagtatt tcgccagact gcgagcgaaa cggaccggat acgggaatcc aaagcgaatt 361 gccggacccg gatatcgttg caaaaggcgc //