LOCUS CZ469259 446 bp DNA linear GSS 16-FEB-2014 DEFINITION c04447 Exelixis piggyBac PB insertions Drosophila melanogaster genomic Sequence recovered from Both 5' and 3' ends of piggyBac, genomic survey sequence. ACCESSION CZ469259 VERSION CZ469259.1 DBLINK BioSample: SAMN00183072 KEYWORDS GSS. SOURCE Drosophila melanogaster (fruit fly) ORGANISM Drosophila melanogaster Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora. REFERENCE 1 (bases 1 to 446) AUTHORS Thibault,S.T., Singer,M.A., Miyazaki,W.Y., Milash,B., Dompe,N.A., Singh,C.M., Buchholz,R., Demsky,M., Fawcett,R., Francis-Lang,H.L., Ryner,L., Cheung,L.M., Chong,A., Erickson,C., Fisher,W.W., Greer,K., Hartouni,S.R., Howie,E., Jakkula,L., Joo,D., Killpack,K., Laufer,A., Mazzotta,J., Smith,R.D., Stevens,L.M., Stuber,C., Tan,L.R., Ventura,R., Woo,A., Zakrajsek,I., Zhao,L., Chen,F., Swimmer,C., Kopczynski,C., Duyk,G., Winberg,M.L. and Margolis,J. TITLE A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac JOURNAL Nat. Genet. 36 (3), 283-287 (2004) PUBMED 14981521 COMMENT Contact: Roger A Hoskins Life Sciences Division Lawrence Berkeley National Laboratory Mailstop 977-0164, One Cyclotron Road, Berkeley, CA 94720, USA Tel: 510 486 4015 Fax: 510 486 6798 Email: rhoskins@lbl.gov Sequence recovery method was inverse PCR. Sequence orientation is forward strand relative to 5' end of piggyBac element. The piggyBac insertion position is 76 in the 446 bases. This insertion position refers to the first base of the 4 base TTAA target recognition sequence. Class: transposon insertion site. FEATURES Location/Qualifiers source 1..446 /organism="Drosophila melanogaster" /mol_type="genomic DNA" /strain="isogenic w- strain" /db_xref="BDGP_INS:c04447" /db_xref="taxon:7227" /clone_lib="SAMN00183072 Exelixis piggyBac PB insertions" /note="Vector: piggyBac PB (GenBank accession number AY515146); An isogenic w- Drosophila melanogaster strain was mutagenized by remobilization of transposable elements. We remobilized the PB element using Hsp70:piggyBac transposase from a single ammunition element on either the X or third chromosome. We induced transposase expression by immersing bottles in a circulating 37oC water bath for a daily (days 3-10 after egg-laying) 1-h heat shock. We outcrossed the resulting dysgenic males to an isogenic w- strain. New insertions were identified on the basis of a change in eye color (third chromosome ammunition) or the appearance of w+ male progeny (X chromosome ammunition). All lines were mapped to a chromosome by standard genetic methods, examined for homozygous viability, and used for recovery of flanking genomic sequence by inverse PCR." BASE COUNT 100 a 128 c 115 g 103 t ORIGIN 1 gatctgccgc ctaccttgtt aacatccgct cccgccttta gcaaggcatt gcagcagtcc 61 aaactccgcg actgcttaac gatggcggcg tgcaacgcgg ttctaccatc tcggtaaacg 121 gcattcacat tggctccgta agatatcagt aaatccacgg tgccgattga ctgcgaaagg 181 caggcgaggt gcaagggcgt ttgatggctg gcgttacggc agtccacctc agcacggtgt 241 tccaggagca agcgagcaca ttctacatag tcgttttcgg cagctgcaat ttgtggattt 301 accaatctat tttgcaaagc acttgtaaac cccttgccac tactcaccca agtgcagggc 361 cgtaaccttt ccctcgccaa actgcgatcg cacgtcggcg ttgtgactga gaagaagctg 421 gacgcatttg gcgaatcctg cggcgc //