LOCUS       CZ469259                 446 bp    DNA     linear   GSS 16-FEB-2014
DEFINITION  c04447 Exelixis piggyBac PB insertions Drosophila melanogaster
            genomic Sequence recovered from Both 5' and 3' ends of piggyBac,
            genomic survey sequence.
ACCESSION   CZ469259
VERSION     CZ469259.1
DBLINK      BioSample: SAMN00183072
KEYWORDS    GSS.
SOURCE      Drosophila melanogaster (fruit fly)
  ORGANISM  Drosophila melanogaster
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Diptera; Brachycera;
            Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Thibault,S.T., Singer,M.A., Miyazaki,W.Y., Milash,B., Dompe,N.A.,
            Singh,C.M., Buchholz,R., Demsky,M., Fawcett,R., Francis-Lang,H.L.,
            Ryner,L., Cheung,L.M., Chong,A., Erickson,C., Fisher,W.W.,
            Greer,K., Hartouni,S.R., Howie,E., Jakkula,L., Joo,D., Killpack,K.,
            Laufer,A., Mazzotta,J., Smith,R.D., Stevens,L.M., Stuber,C.,
            Tan,L.R., Ventura,R., Woo,A., Zakrajsek,I., Zhao,L., Chen,F.,
            Swimmer,C., Kopczynski,C., Duyk,G., Winberg,M.L. and Margolis,J.
  TITLE     A complementary transposon tool kit for Drosophila melanogaster
            using P and piggyBac
  JOURNAL   Nat. Genet. 36 (3), 283-287 (2004)
   PUBMED   14981521
COMMENT     Contact: Roger A Hoskins
            Life Sciences Division
            Lawrence Berkeley National Laboratory
            Mailstop 977-0164, One Cyclotron Road, Berkeley, CA 94720, USA
            Tel: 510 486 4015
            Fax: 510 486 6798
            Email: rhoskins@lbl.gov
            
            Sequence recovery method was inverse PCR.
            Sequence orientation is forward strand relative to 5' end of
            piggyBac element.
            The piggyBac insertion position is 76 in the 446 bases. This
            insertion position refers to the first base of the 4 base TTAA
            target recognition sequence.
            Class: transposon insertion site.
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Drosophila melanogaster"
                     /mol_type="genomic DNA"
                     /strain="isogenic w- strain"
                     /db_xref="BDGP_INS:c04447"
                     /db_xref="taxon:7227"
                     /clone_lib="SAMN00183072 Exelixis piggyBac PB insertions"
                     /note="Vector: piggyBac PB (GenBank accession number
                     AY515146); An isogenic w- Drosophila melanogaster strain
                     was mutagenized by remobilization of transposable
                     elements. We remobilized the PB element using
                     Hsp70:piggyBac transposase from a single ammunition
                     element on either the X or third chromosome. We induced
                     transposase expression by immersing bottles in a
                     circulating 37oC water bath for a daily (days 3-10 after
                     egg-laying) 1-h heat shock. We outcrossed the resulting
                     dysgenic males to an isogenic w- strain. New insertions
                     were identified on the basis of a change in eye color
                     (third chromosome ammunition) or the appearance of w+ male
                     progeny (X chromosome ammunition). All lines were mapped
                     to a chromosome by standard genetic methods, examined for
                     homozygous viability, and used for recovery of flanking
                     genomic sequence by inverse PCR."
BASE COUNT          100 a          128 c          115 g          103 t
ORIGIN      
        1 gatctgccgc ctaccttgtt aacatccgct cccgccttta gcaaggcatt gcagcagtcc
       61 aaactccgcg actgcttaac gatggcggcg tgcaacgcgg ttctaccatc tcggtaaacg
      121 gcattcacat tggctccgta agatatcagt aaatccacgg tgccgattga ctgcgaaagg
      181 caggcgaggt gcaagggcgt ttgatggctg gcgttacggc agtccacctc agcacggtgt
      241 tccaggagca agcgagcaca ttctacatag tcgttttcgg cagctgcaat ttgtggattt
      301 accaatctat tttgcaaagc acttgtaaac cccttgccac tactcaccca agtgcagggc
      361 cgtaaccttt ccctcgccaa actgcgatcg cacgtcggcg ttgtgactga gaagaagctg
      421 gacgcatttg gcgaatcctg cggcgc
//