LOCUS CZ466345 195 bp DNA linear GSS 16-FEB-2014 DEFINITION c00284-5prime Exelixis piggyBac PB insertions Drosophila melanogaster genomic Sequence recovered from 5' end of piggyBac, genomic survey sequence. ACCESSION CZ466345 VERSION CZ466345.1 DBLINK BioSample: SAMN00183072 KEYWORDS GSS. SOURCE Drosophila melanogaster (fruit fly) ORGANISM Drosophila melanogaster Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora. REFERENCE 1 (bases 1 to 195) AUTHORS Thibault,S.T., Singer,M.A., Miyazaki,W.Y., Milash,B., Dompe,N.A., Singh,C.M., Buchholz,R., Demsky,M., Fawcett,R., Francis-Lang,H.L., Ryner,L., Cheung,L.M., Chong,A., Erickson,C., Fisher,W.W., Greer,K., Hartouni,S.R., Howie,E., Jakkula,L., Joo,D., Killpack,K., Laufer,A., Mazzotta,J., Smith,R.D., Stevens,L.M., Stuber,C., Tan,L.R., Ventura,R., Woo,A., Zakrajsek,I., Zhao,L., Chen,F., Swimmer,C., Kopczynski,C., Duyk,G., Winberg,M.L. and Margolis,J. TITLE A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac JOURNAL Nat. Genet. 36 (3), 283-287 (2004) PUBMED 14981521 COMMENT Contact: Roger A Hoskins Life Sciences Division Lawrence Berkeley National Laboratory Mailstop 977-0164, One Cyclotron Road, Berkeley, CA 94720, USA Tel: 510 486 4015 Fax: 510 486 6798 Email: rhoskins@lbl.gov Sequence recovery method was inverse PCR. Sequence orientation is forward strand relative to 5' end of piggyBac element. The piggyBac insertion position is 192 in the 195 bases. This insertion position refers to the first base of the 4 base TTAA target recognition sequence. Class: transposon insertion site. FEATURES Location/Qualifiers source 1..195 /organism="Drosophila melanogaster" /mol_type="genomic DNA" /strain="isogenic w- strain" /db_xref="BDGP_INS:c00284" /db_xref="taxon:7227" /clone_lib="SAMN00183072 Exelixis piggyBac PB insertions" /note="Vector: piggyBac PB (GenBank accession number AY515146); An isogenic w- Drosophila melanogaster strain was mutagenized by remobilization of transposable elements. We remobilized the PB element using Hsp70:piggyBac transposase from a single ammunition element on either the X or third chromosome. We induced transposase expression by immersing bottles in a circulating 37oC water bath for a daily (days 3-10 after egg-laying) 1-h heat shock. We outcrossed the resulting dysgenic males to an isogenic w- strain. New insertions were identified on the basis of a change in eye color (third chromosome ammunition) or the appearance of w+ male progeny (X chromosome ammunition). All lines were mapped to a chromosome by standard genetic methods, examined for homozygous viability, and used for recovery of flanking genomic sequence by inverse PCR." BASE COUNT 51 a 44 c 24 g 76 t ORIGIN 1 gatcactcca ctgatttatg cccttcttga aagattccac tcccaattcg aactgctcat 61 tgtgttcctg aatcgctttc caattgtcgc aaaatatagt tcggcgtttt tctgtttctt 121 tttcatcttg atatttcgca ccgaagtcaa tctgtaataa agatatattc attcaaattc 181 tcaaattcta tttaa //