LOCUS CV758690 874 bp mRNA linear EST 09-NOV-2004
DEFINITION FGAS052966 Triticum aestivum FGAS: Library 2 Gate 3 Triticum
aestivum cDNA, mRNA sequence.
ACCESSION CV758690
VERSION CV758690.1
DBLINK BioSample: SAMN00154148
KEYWORDS EST.
SOURCE Triticum aestivum (bread wheat)
ORGANISM Triticum aestivum
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP
clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
REFERENCE 1 (bases 1 to 874)
AUTHORS Allard,F., Crosby,W.L., Danyluk,J., Eudes,F., Frick,M., Gaudet,D.,
Genswein,B., Graf,R., Gulick,P., Hrycan,L.D., Laroche,A.,
Links,M.G., McCarthy,E.L., Monroy,A., Muzak,I., Nilson,D.,
Penniket,C., Roach,J.L. and Sarhan,F.
TITLE Functional Genomics of Abiotic Stress In Wheat and Canola Crops
JOURNAL Unpublished
COMMENT Contact: Wm L Crosby
Bioinformatics
University of Saskatchewan, Department of Computer Science
1C101 Engineering Building, 57 Campus Drive, Saskatoon,
Saskatchewan, S7N 5A9, Canada
Tel: 306 966 1769
Fax: 306 966 2033
Email: fgas_ests@cs.usask.ca
This sequence is the direct result of the Base calling software
Phred (default parameters). It is the raw base calls. To aid in the
identification of the high quality insert the software Lucy
(default parameters) has been run on this sequence. Lucy identified
the region [99,583].
Plate: WEF001 row: E column: 11.
FEATURES Location/Qualifiers
source 1..874
/organism="Triticum aestivum"
/mol_type="mRNA"
/db_xref="taxon:4565"
/clone_lib="SAMN00154148 Triticum aestivum FGAS: Library 2
Gate 3"
/note="Organ: Crown and leaf; Vector: pCMV.SPORT6; Aerial
parts (crown and leaf) of wheat cultivar Norstar from
control and long exposure times to low temperature. 4 mRNA
populations were combined before constructing the library;
7 days non-acclimated plants and 1, 23, and 53 days
cold-acclimated at 4C. Non-acclimated and cold-acclimated
plants were grown in vermiculite This is the only library
that was done according to the Invitrogen manual, and
therefore, a percentage of clones will not have the 3
prime end because of NotI digestion within the cDNA."
BASE COUNT 153 a 307 c 280 g 134 t
ORIGIN
1 acgctatgac cattaggcct atttaggtga cacttcgaac agaggtgtac aaaaaagcag
61 gtctgctacc ggtccggaat tcccgggata tcgtcgaccc acgcgtccgg gccgagagaa
121 aaagcttggc tgagaatcgc ctggtcactg cagccaatca cggtggtggc gctggtgcta
181 gctgggctcg tcggactgtg agcgagcagt gagcgcgtac gtgccatgga gggggaggag
241 aagccggtgg tgggcggggc ctactggggc gtgggcgcga gggcctgcga ctcgtgcgcc
301 accgaggcgg ccaggctctt ctgccgcgcc gacgccgcgt tcctctgcgc cggctgcgac
361 gcgcgcgcgc acggcgccgg gtcgcgccac gcccgggtct ggctctgcga ggtctgcgag
421 cacgcgcccg cggccgtcac gtgcaaggcc gacgccgccg tgctctgcgc ctcctgcgac
481 gccgacatcc acgccgccaa cccgctcgcg cgccgacacg agcgcgtccc cgtcgcgccc
541 ttcttcggca cggccgccga cgcgcacaag cccttcgtgt cgtcgggagc ccaggccgct
601 gcccgagacg acgggagcaa cgacgccgaa ggggcgtcgt ggctgctccc caagcccgat
661 cacaaagatt ggtgctaccc gccgaagtct tctttcgcgg attccgaacc attaccctcg
721 acctcgactt ccccgcgggt caatgggcac aacttcaagg gccatcagcg gtgcagcttc
781 aacggcccag ccccgaaaat cgaacctcca acggcgggaa ccaaagaggt tttcaacttc
841 caaccccctc catggaaccc acaccggtat ccgt
//