LOCUS CR533470 792 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834D0517D for
gene CLDN12, claudin 12; complete cds, incl. stopcodon.
ACCESSION CR533470
VERSION CR533470.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 735)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 735)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (21-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834D0517D, ORFNo 2785
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834D0517D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full ORF clones
generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
After the stop codon 3' UTR sequence is present in front of the
3' att site (ACCCAGCTTTCTT).
Compared to the reference sequence NM_012129 (gi37537523)
we found AA exchange(s) at position (first base of changed
triplet):
67(gly->cys) 133(glu->gly) 682(ser->pro)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..792
/db_xref="H-InvDB:HIT000268318"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834D0517D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..735
/codon_start=1
/gene="CLDN12"
/db_xref="GOA:Q6FIF6"
/db_xref="H-InvDB:HIT000268318.12"
/db_xref="InterPro:IPR013287"
/db_xref="InterPro:IPR017974"
/db_xref="UniProtKB/TrEMBL:Q6FIF6"
/protein_id="CAG38501.1"
/translation="MGCRDVHAATVLSFLCGIASVACLFAGTLLPNWRKLRLITFNRN
GKNLTVYTGLWVKCARYDGSSDCLMYDTTWYSSVDQLDLRVLQFALPLSMLIAMGALL
LCLIGMCNTAFRSSVPNIKLAKCLVNSAGCHLVAGLLFFLAGTVSLSPSIWVIFYNIH
LNKKFEPVFSFDYAVYVTIASAGGLFMTSLILFIWYCTCKSLPSPFWQPLYSHPPSMH
TYSQPYSARPRLSAIEIDIPVVSHTT"
BASE COUNT 170 a 216 c 172 g 234 t
ORIGIN
1 atgggctgtc gggatgtcca cgcagccaca gtcctttcct tcctgtgtgg aatcgcctca
61 gtagcatgcc tctttgcagg gactctgctt cccaactgga gaaaattacg attgatcaca
121 ttcaacagaa acgggaagaa cctgactgtt tacacaggcc tgtgggtgaa atgtgcccgg
181 tatgacggga gcagtgactg cctgatgtac gacactactt ggtactcatc agttgaccag
241 ctggacctgc gtgtcctcca gtttgcccta cccctcagca tgctgatcgc catgggtgcc
301 ctgctgctct gcctgattgg aatgtgcaac actgccttca ggtcctcggt gcccaacatc
361 aaactggcca agtgtctggt caatagtgca ggttgccacc tggtggctgg gctgctattt
421 ttcctggcag gtactgtgag cctctcccca tctatctggg tcatctttta taacatccat
481 ctgaacaaga agtttgagcc agtcttttca tttgactatg cagtgtatgt cactattgct
541 agtgctgggg gcctgtttat gacttccctt atactattta tttggtattg tacatgcaaa
601 tctttgcctt ctcctttctg gcaaccattg tactcccatc cacccagtat gcatacttac
661 tcacagccct attcagcacg ccctcgcctc tctgccattg aaattgacat tccagtagtt
721 tcacacacca cttaatgggg aaatagttaa ttgttaaaga aaacttcttg tagcctcaca
781 ttccccttgt gc
//