LOCUS CR457305 1185 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834A1213D for
gene PHAX, likely ortholog of mouse phosphorylated adaptor for RNA
export; complete cds, incl. stopcodon.
ACCESSION CR457305
VERSION CR457305.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1185)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1185)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834A1213D, ORFNo 2340
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834A1213D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence BC021161 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1185
/db_xref="H-InvDB:HIT000268155"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834A1213D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1185
/codon_start=1
/gene="PHAX"
/db_xref="GOA:Q9H814"
/db_xref="H-InvDB:HIT000268155.13"
/db_xref="HGNC:HGNC:10241"
/db_xref="InterPro:IPR019385"
/db_xref="InterPro:IPR038092"
/db_xref="InterPro:IPR039047"
/db_xref="PDB:2XC7"
/db_xref="UniProtKB/Swiss-Prot:Q9H814"
/protein_id="CAG33586.1"
/translation="MALEVGDMEDGQLSDSDSDMTVAPSDRPLQLPKVLGGDSAMRAF
QNTATACAPVSHYRAVESVDSSEESFSDSDDDSCLWKRKRQKCFNPPPKPEPFQFGQS
SQKPPVAGGKKINNIWGAVLQEQNQDAVATELGILGMEGTIDRSRQSETYNYLLAKKL
RKESQEHTKDLDKELDEYMHGGKKMGSKEEENGQGHLKRKRPVKDRLGNRPEMNYKGR
YEITAEDSQEKVADEISFRLQEPKKDLIARVVRIIGNKKAIELLMETAEVEQNGGLFI
MNGSRRRTPGGVFLNLLKNTPSISEEQIKDIFYIENQKEYENKKAARKRRTQVLGKKM
KQAIKSLNFQEDDDTSRETFASDTNEALASLDESQEGHAEAKLEAEEAIEVDHSHDLD
IF"
BASE COUNT 415 a 210 c 300 g 260 t
ORIGIN
1 atggcgttgg aggtcggcga tatggaagat gggcagcttt ccgactcgga ttccgacatg
61 acggtcgcac ccagcgacag gccgctgcaa ttgccaaaag tgctaggtgg cgacagtgct
121 atgagggcct tccagaacac ggcaactgca tgtgcaccag tatcacatta tcgagctgtt
181 gaaagtgtgg attcaagtga agaaagtttt tctgattcag atgatgatag ctgtctttgg
241 aaacgcaaac gacagaaatg ttttaaccct cctcccaaac cagagccttt tcagtttggc
301 cagagcagtc agaaaccacc tgttgctgga ggaaagaaga ttaacaacat atggggtgct
361 gtgctgcagg aacagaatca agatgcagtg gccactgaac ttggtatctt gggaatggag
421 ggcactattg acagaagcag acaatccgag acctacaatt atttgcttgc caagaaactt
481 aggaaggaat ctcaagagca tacaaaagat ctagacaagg aactagatga atatatgcat
541 ggtggcaaaa aaatgggatc aaaggaagag gaaaatgggc aaggtcatct caaaaggaaa
601 cgacctgtca aagacaggct agggaacaga ccagaaatga actataaagg tcgatacgag
661 atcacagcgg aagattctca agagaaagtg gctgatgaaa tttcattcag gttacaggaa
721 ccaaagaaag acctgatagc ccgagtagtg aggattattg gtaacaaaaa ggcaattgaa
781 cttctgatgg aaaccgctga agttgaacaa aatggtggtc tctttataat gaatggtagt
841 cgaagaagaa caccaggtgg agtttttcta aatctcttga aaaacactcc tagtatcagc
901 gaggaacaaa ttaaggacat tttctacatt gaaaaccaaa aggaatatga aaataaaaaa
961 gctgctagga agaggagaac acaagtgttg gggaaaaaga tgaaacaagc tattaaaagt
1021 ctaaattttc aagaagatga tgatacatca cgagaaactt ttgcaagtga cacgaatgag
1081 gccttggcct ctcttgatga gtcacaggaa ggacatgcag aagccaagtt ggaggcagag
1141 gaagccattg aagttgatca ttctcatgat ttggacatct tttaa
//