LOCUS       CR457226                 975 bp    mRNA    linear   HUM 16-OCT-2008
DEFINITION  Homo sapiens full open reading frame cDNA clone RZPDo834H0412D for
            gene TRIT1, tRNA isopentenyltransferase 1; complete cds, incl.
            stopcodon.
ACCESSION   CR457226
VERSION     CR457226.1
KEYWORDS    Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
  TITLE     Cloning of human full open reading frames in Gateway(TM) system
            entry vector (pDONR201)
  JOURNAL   Unpublished.
REFERENCE   2  (bases 1 to 975)
  AUTHORS   Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
  JOURNAL   Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
            Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
            580, D-69120 Heidelberg, Germany
COMMENT     RZPD; RZPDo834H0412D, ORFNo 2121
            www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834H0412D
            RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
            RZPD LIB No. 834
            www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
            www.rzpd.de/products/orfclones/
            Contact: Inge Arlart
            RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
            Heubnerweg 6, D-14059 Berlin, Germany
            Tel: +49 30 32639 100
            Fax: +49 30 32639 111
            www.rzpd.de
            This clone is available from RZPD;
            contact RZPD (customer.service@rzpd.de) for further information.
            
            This CDS clone is a part of a collection of human full length
            expression clones generated by RZPD.
            This CDS has been cloned incl. stopcodon.
            The CDS has been inserted into pDONR201 via a BP Clonase(TM)
            reaction.
            Additional sequence has been added in front of the start codon:
            att..AAAAAA GCA GGC (ATG).
            The last base of the last coding triplett has been changed to T,
            which might lead to an amino acid change at the C terminus of the
            polypeptide.
            The stop codon has been set to TAA followed by
            TTAACCCAGCTTTCTT..att.
            Compared to the reference sequence BC010741 we found amino acid
            exchange(s) at position (first base of changed triplet):
            478(ser->gly)
            Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES             Location/Qualifiers
     source          1..975
                     /db_xref="H-InvDB:HIT000268076"
                     /organism="Homo sapiens"
                     /lab_host="DH10B"
                     /mol_type="mRNA"
                     /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
                     /clone="RZPDo834H0412D"
                     /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
                     /db_xref="taxon:9606"
     CDS             1..975
                     /codon_start=1
                     /gene="TRIT1"
                     /db_xref="GOA:Q9H3H1"
                     /db_xref="H-InvDB:HIT000268076.13"
                     /db_xref="HGNC:HGNC:20286"
                     /db_xref="InterPro:IPR003604"
                     /db_xref="InterPro:IPR018022"
                     /db_xref="InterPro:IPR022755"
                     /db_xref="InterPro:IPR027417"
                     /db_xref="InterPro:IPR030666"
                     /db_xref="InterPro:IPR036236"
                     /db_xref="InterPro:IPR039657"
                     /db_xref="UniProtKB/Swiss-Prot:Q9H3H1"
                     /protein_id="CAG33507.1"
                     /translation="MGTEKVIDRKVELEKEDGLVLHKRLSQVDPEMAAKLHPHDKRKV
                     ARSLQVFEETGISHSEFLHRQHTEEGGGPLGGPLKFSNPCILWLHADQADERLDKRVD
                     DMLAAGLLEELRDFHRRYNQKNVSENSQDYQHGIFQSIGFKEFHEYLITEGKCTLETG
                     NQLLKKGIEALKQVTKRYARKQNRWVKNRFLSRPGPIVPPVYGLEVSDVSKWEESVLE
                     PALEIVQSFIQGHKPTATPIKMPYNEAENKRSYHLCDLCDRIIIGDREWAAHIKSKSH
                     LNQLKKRRRLDSDAVNTIESQSVSPDHNKEPKEKGSPGQNDQELKCSV"
BASE COUNT          307 a          210 c          238 g          220 t
ORIGIN      
        1 atgggcactg agaaagtgat tgaccgaaaa gtggagcttg aaaaggagga tggtcttgta
       61 cttcacaaac gcctaagcca ggtggaccca gaaatggctg ccaagctgca tccacatgac
      121 aaacgcaaag tggccaggag cttgcaagtt tttgaagaaa caggaatctc tcatagtgaa
      181 tttctccatc gtcaacatac ggaagaaggt ggtggtcccc ttggaggtcc tctgaagttc
      241 tctaaccctt gcatcctttg gcttcatgct gaccaggcag atgagcgctt ggataagagg
      301 gtggatgaca tgcttgctgc tgggctcttg gaggaactaa gagattttca cagacgctat
      361 aatcagaaga atgtttcgga aaatagccag gactatcaac atggtatctt ccaatcaatt
      421 ggcttcaagg aatttcacga gtacctgatc actgagggaa aatgcacact ggagactggt
      481 aaccagcttc taaagaaagg tattgaggct ctgaaacaag taactaagag atatgcccgg
      541 aaacaaaacc gatgggttaa aaaccgtttt ttgagcagac ctggtcccat tgtcccccct
      601 gtctatggct tagaggtatc tgatgtctcg aagtgggaag agtctgttct tgaacctgct
      661 cttgaaatcg tgcaaagttt catccagggc cacaagccta cagccactcc aataaagatg
      721 ccatacaatg aagctgagaa caagagaagt tatcacctgt gtgacctctg tgatcgaatc
      781 atcattgggg atcgcgaatg ggcagcgcac ataaaatcca aatcccactt gaaccaactg
      841 aagaaaagaa gaagattgga ctcagatgct gtcaacacca tagaaagtca gagtgtttcc
      901 ccagaccata acaaagaacc taaagagaag ggatccccag ggcagaatga tcaagagctg
      961 aaatgcagcg tttaa
//