LOCUS CR457226 975 bp mRNA linear HUM 16-OCT-2008 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834H0412D for gene TRIT1, tRNA isopentenyltransferase 1; complete cds, incl. stopcodon. ACCESSION CR457226 VERSION CR457226.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 975) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 975) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834H0412D, ORFNo 2121 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834H0412D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Inge Arlart RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 100 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; contact RZPD (customer.service@rzpd.de) for further information. This CDS clone is a part of a collection of human full length expression clones generated by RZPD. This CDS has been cloned incl. stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon: att..AAAAAA GCA GGC (ATG). The last base of the last coding triplett has been changed to T, which might lead to an amino acid change at the C terminus of the polypeptide. The stop codon has been set to TAA followed by TTAACCCAGCTTTCTT..att. Compared to the reference sequence BC010741 we found amino acid exchange(s) at position (first base of changed triplet): 478(ser->gly) Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..975 /db_xref="H-InvDB:HIT000268076" /organism="Homo sapiens" /lab_host="DH10B" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834H0412D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..975 /codon_start=1 /gene="TRIT1" /db_xref="GOA:Q9H3H1" /db_xref="H-InvDB:HIT000268076.13" /db_xref="HGNC:HGNC:20286" /db_xref="InterPro:IPR003604" /db_xref="InterPro:IPR018022" /db_xref="InterPro:IPR022755" /db_xref="InterPro:IPR027417" /db_xref="InterPro:IPR030666" /db_xref="InterPro:IPR036236" /db_xref="InterPro:IPR039657" /db_xref="UniProtKB/Swiss-Prot:Q9H3H1" /protein_id="CAG33507.1" /translation="MGTEKVIDRKVELEKEDGLVLHKRLSQVDPEMAAKLHPHDKRKV ARSLQVFEETGISHSEFLHRQHTEEGGGPLGGPLKFSNPCILWLHADQADERLDKRVD DMLAAGLLEELRDFHRRYNQKNVSENSQDYQHGIFQSIGFKEFHEYLITEGKCTLETG NQLLKKGIEALKQVTKRYARKQNRWVKNRFLSRPGPIVPPVYGLEVSDVSKWEESVLE PALEIVQSFIQGHKPTATPIKMPYNEAENKRSYHLCDLCDRIIIGDREWAAHIKSKSH LNQLKKRRRLDSDAVNTIESQSVSPDHNKEPKEKGSPGQNDQELKCSV" BASE COUNT 307 a 210 c 238 g 220 t ORIGIN 1 atgggcactg agaaagtgat tgaccgaaaa gtggagcttg aaaaggagga tggtcttgta 61 cttcacaaac gcctaagcca ggtggaccca gaaatggctg ccaagctgca tccacatgac 121 aaacgcaaag tggccaggag cttgcaagtt tttgaagaaa caggaatctc tcatagtgaa 181 tttctccatc gtcaacatac ggaagaaggt ggtggtcccc ttggaggtcc tctgaagttc 241 tctaaccctt gcatcctttg gcttcatgct gaccaggcag atgagcgctt ggataagagg 301 gtggatgaca tgcttgctgc tgggctcttg gaggaactaa gagattttca cagacgctat 361 aatcagaaga atgtttcgga aaatagccag gactatcaac atggtatctt ccaatcaatt 421 ggcttcaagg aatttcacga gtacctgatc actgagggaa aatgcacact ggagactggt 481 aaccagcttc taaagaaagg tattgaggct ctgaaacaag taactaagag atatgcccgg 541 aaacaaaacc gatgggttaa aaaccgtttt ttgagcagac ctggtcccat tgtcccccct 601 gtctatggct tagaggtatc tgatgtctcg aagtgggaag agtctgttct tgaacctgct 661 cttgaaatcg tgcaaagttt catccagggc cacaagccta cagccactcc aataaagatg 721 ccatacaatg aagctgagaa caagagaagt tatcacctgt gtgacctctg tgatcgaatc 781 atcattgggg atcgcgaatg ggcagcgcac ataaaatcca aatcccactt gaaccaactg 841 aagaaaagaa gaagattgga ctcagatgct gtcaacacca tagaaagtca gagtgtttcc 901 ccagaccata acaaagaacc taaagagaag ggatccccag ggcagaatga tcaagagctg 961 aaatgcagcg tttaa //