LOCUS       CO142321                 503 bp    mRNA    linear   EST 09-FEB-2011
DEFINITION  EST836992 Aspergillus flavus Normalized cDNA Expression Library
            Aspergillus flavus cDNA clone NAFEO39 5' end similar to Glandular
            kallikrein 9 submandibular precursor ()(Tissue kallikrein) (S3
            kallikrein), mRNA sequence.
ACCESSION   CO142321
VERSION     CO142321.1
DBLINK      BioSample: LIBEST_015900
KEYWORDS    EST.
SOURCE      Aspergillus flavus
  ORGANISM  Aspergillus flavus
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae;
            Aspergillus.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Yu,J., Whitelaw,C.A., Nierman,W.C., Bhatnagar,D. and Cleveland,T.E.
  TITLE     Aspergillus flavus expressed sequence tags for identification of
            genes with putative roles in aflatoxin contamination of crops
  JOURNAL   FEMS Microbiol. Lett. 237 (2), 333-340 (2004)
   PUBMED   15321681
COMMENT     Contact: Yu J
            Food and Feed Safety Research Unit
            USDA/ARS, Southern Regional Research Center
            1100 Robert E. Lee Boulevard, New Orleans, LA 70124, USA
            Tel: 504 286 4405
            Fax: 504 286 4419
            Email: jiuyu@srrc.ars.usda.gov
            Contact Dr. Yu at USDA/ARS SRRC (jiuyu@srrc.ars.usda.gov) for clone
            information
            PCR PRimers
            FORWARD: M13F
            BACKWARD: M13R
            Seq primer: M13 Forward
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Aspergillus flavus"
                     /mol_type="mRNA"
                     /strain="NRRL 3357"
                     /db_xref="taxon:5059"
                     /clone="NAFEO39"
                     /sex="asexual mycelia"
                     /cell_type="mycelia"
                     /dev_stage="developmental stages from 18 to 96 hours"
                     /lab_host="E. coli DH10B T1 resistant cells"
                     /clone_lib="LIBEST_015900 Aspergillus flavus Normalized
                     cDNA Expression Library"
                     /note="Vector: pBlueScript (SK+) (Stratagene), antibiotic
                     selection marker: Carbenicillin; Site_1: NotI, at the 5
                     prime end; Site_2: EcoRI, at the 3 prime end; This
                     normalized cDNA expression library was constructed using a
                     mixture of mycelial cells grown under eight different
                     medium conditions and harvested at 5 time points (18, 24,
                     48, 72, 96 hours). The poly-A sequence was trimmed off
                     before ligating to vector."
BASE COUNT          126 a          142 c          120 g          115 t
ORIGIN      
        1 tcctggacag tctcgggttg ttgcgacgat ataactgtga gacgaattcc caaccctggc
       61 aagtggctgt cattggtaca actttctgcg ggggtgtcct gatagacccc agctgggtga
      121 tcacagctgc ccactgctat agcaagaatt atcgtgattt gctgggccga aacgacctag
      181 ttaaagatga accctttgct caacgccggc ttgtcagcca aagcttccaa caccctgact
      241 acatcccagt cttcatgagg aaccacaccc gaccacgtgc gtatgaccac aacaatgacc
      301 tgatgctgct ccacctcagc aagtctgctg acatcacagg tggtgtgaag gtcatcgatc
      361 tgcccactga ggagcccaaa gtggggagca tctgccttgc ctcaagctgg ggcatgacca
      421 acccctctga gatgaaatta agtcatgatc tccagtgtgt gaacatccac cttctgtcta
      481 atgagaagtg catcgaaacc tac
//