LOCUS CK335235 585 bp mRNA linear EST 12-MAY-2010 DEFINITION H3154E06-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3154E06 5', mRNA sequence. ACCESSION CK335235 VERSION CK335235.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 585) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3154 row: E column: 06 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..585 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3154E06-5" /db_xref="taxon:10090" /clone="H3154E06" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 112 a 184 c 176 g 113 t ORIGIN 1 gaggagcctc ttagcctctt ggacgacgtg aaccactgct actcgcgcct gcgggaactg 61 gtgccgggag tcccgcgagg cactcagctt agccaggtgg aaatcctgca gcgtgtcata 121 gactacatcc tcgaccttca ggtggtcctg gcagagccgg cgcctggacc cccggacggt 181 ccgcatctcc cgatccagac agctgagctc actccggaac ttgtgatctc caaggacaag 241 aggagctttt gccactgacc cggtcgtcct ggcacctccc gaacgcaggt gctggcgccc 301 gttccgcttg ggaccctggg actctgggac cctctctcca gccggaagcc tgagggcatg 361 gatgagcttc gatcttaacc cagccctctt cacttaccct gaactcaacg cctcgaggct 421 ggacctggag cccgagagaa ggactgaact tgggtggcct gaagagctag cacacgctgg 481 tcagcagctg ggcaacgtca ctctgtcccc accctgactc aagtctaaaa gactggcttt 541 tccgagaatg gggtgtccag agggtgtggg gggatgcgag tggct //