LOCUS       CK335191                 586 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3151A02-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3151A02 5', mRNA sequence.
ACCESSION   CK335191
VERSION     CK335191.1
DBLINK      BioSample: LIBEST_008622
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     Contact: Dawood B. Dudekula
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            Plate: H3151  row: A  column: 02
            Seq primer: M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3151A02-5"
                     /db_xref="taxon:10090"
                     /clone="H3151A02"
                     /sex="Clones arrayed from a variety of cDNA libraries"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /clone_lib="LIBEST_008622 NIA Mouse 15K cDNA Clone Set"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo  cDNA libraries (including preimplantation stage
                     embryos from  unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos,  extraembryonic part of E7.5
                     embryos, and E12.5 female mesonephros/gonad)  and one
                     newborn ovary cDNA library. Average insert size 1.5 kb.
                     All  source libraries are cloned unidirectionally with
                     Oligo(dT)-Not primers.  References include: (1)
                     Genome-wide expression profiling of mid-gestation
                     placenta and embryo using a 15,000 mouse developmental
                     cDNA microarray,  2000, Proc. Natl. Acad. Sci. U S A, 97:
                     9127-9132; (2) Large-scale cDNA  analysis reveals phased
                     gene expression patterns during preimplantation  mouse
                     develolpment, 2000, Development, 127: 1737-1749; (3)
                     Genome-wide  mapping of unselected transcripts from
                     extraembryonic tissue of 7.5-day  mouse embryos reveals
                     enrichment in the t-complex and under-representation  on
                     the X chromosome, 1998, Hum Mol Genet 7: 1967-1978."
BASE COUNT          141 a          134 c          170 g          141 t
ORIGIN      
        1 taaagtgaaa gttaaccgcc tctttgaagc ccaaggaaaa ccggagctga aaggttttaa
       61 cctgaatccc ctcagcccgg atgaggttaa agccctcaag gtcatcttga aaggatgaga
      121 ctcacagaag aatgggggac ctgtggtagg acttcccaac tcccagcctt gagacctgca
      181 agggcaggat tgtctgttta aggggacagg catcaggaat gtggcttctg tacaaatctt
      241 tttctgtagt gcttggttgc tttgtgcaag agagaaccct tagtctgttc tgtgggcttg
      301 gcctggtcac cagagcttgg acacaagacc tattgacaaa ccctggcagc tgtcagtagg
      361 tgtgtgggtg tgtgagaatg tctgcagccg acacttctca acagtagcct gctgttcaca
      421 tgggcctggc cggggatgga gggaggggaa ggtggactgg gagcttcatt ccagctttct
      481 gcacagcgag gcattttcag gcagtgtgtg tgacaactgg aataaagcag gacactgacc
      541 gttcacttct gcgtgaagca gacctagtgc tcctcaccag cttcag
//