LOCUS CK334925 597 bp mRNA linear EST 12-MAY-2010 DEFINITION H3123G12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3123G12 5', mRNA sequence. ACCESSION CK334925 VERSION CK334925.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 597) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3123 row: G column: 12 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..597 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3123G12-5" /db_xref="taxon:10090" /clone="H3123G12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 154 a 140 c 161 g 142 t ORIGIN 1 gggagaggcg gcagtcggca gcgcaactgt cggcacagca gcccggcggc cccgaaaggt 61 cccaggtatc acgctcatca ggtagttcag ctcacctgtg aaactgagaa ccctggatgg 121 tgaagagagg aaactagcaa aggatccaga cactttgtct aggtagtcct ggaagttctg 181 gaatttagaa aataagccag tgcttgaact gagacctccg ctgccctagc ctctccagtg 241 ctggggtaaa aggcttgcac cacctggcct ttctgacttg ttctgacatg accacacttg 301 agacttctgc atgaggtttg agaagattgt tcttctcctg tcctgtggat ggttttccag 361 aggtctctaa ctgctcagtc agatctgctc acctgttaat cagaactaag agccctggat 421 ggtgaacaca gaggaaacta tagccaagga accaggtaag agtaactaga agaatgaacg 481 cctctctcct aaatgctcct cagggtcagt taacattcaa ggatgtggct gttgacttct 541 cacaggagga atgggaatgt cttgactgtg ctcagagggc attgtacatg gatgtga //