LOCUS CK334610 548 bp mRNA linear EST 12-MAY-2010 DEFINITION H3064C03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3064C03 3', mRNA sequence. ACCESSION CK334610 VERSION CK334610.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 548) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3064 row: C column: 03 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..548 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3064C03-3" /db_xref="taxon:10090" /clone="H3064C03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 159 a 107 c 118 g 164 t ORIGIN 1 cataaaaatt gggttttaaa aagactaaga tttaacccaa ggggttgaat tttacaaaag 61 tgagaatggt acaaacccta aatggaatga cctcagtgct gggagaacat gtccatgtga 121 acctgctggg tatattaacc atcagtctta aggaggcagc aggactggtc tccagcagga 181 cttggttttg gatccattgg aacttgccag tgtacctgta tgtagttggc agccattctt 241 gcacactgtt ggcctctctc agagcttgac aaagtgcaac ttttacagtt gtcacatctg 301 ctttggactg ttcagtatgc ccgcattact tctgaaagaa cacgtcccat gggtctccct 361 ggcttttaat actgtgtgat cactcttgga aaacgttaac agattatgac aattactaag 421 ataatacatc aagcatagtt atgtggagac attggtagca aggctgtatt caacatatga 481 ccctatctgg ttccataata gtactgggta ccttgcctaa ctatgtgttt taaagtaata 541 atatgcat //