LOCUS CK334362 631 bp mRNA linear EST 12-MAY-2010 DEFINITION H3019E05-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3019E05 5', mRNA sequence. ACCESSION CK334362 VERSION CK334362.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 631) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3019 row: E column: 05 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..631 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3019E05-5" /db_xref="taxon:10090" /clone="H3019E05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 180 a 165 c 158 g 128 t ORIGIN 1 atcttcatcg ggaccatcca agtaatgctg ctaaccatca gtgaactgaa gcccacatac 61 caggaccgag tggccccgcc aggattgaca cagattcccc agatccagaa gactgagatc 121 tccttccgtc ctaatgaccc caagagctac gaggcctacg tgctaaacat catcaggttc 181 ctggaaaagt acaaggattc agcccagaag gacgacatga ttttcgagga ctgtggcaat 241 gttcccagtg aacccaagga acggggcgac atcaatcacg aacgaggaga gaggaaggtg 301 tgcaggttca agcttgactg gctggggaac tgctccggtc tcaatgatga ctcttacggc 361 tacagagagg ggaagccctg catcattatc aagctcaacc gagtgttggg cttcaaaccg 421 aagcctccca agaatgaatc cttggagact tacccactga tgatgaagta taatccaaat 481 gtcctgcctg ttcagtgcac tggcaagaga gatgaagata aggataaagt cgggaacata 541 gagtactttg ggatgggcgg atactacggc ttccctctgc agtattatcc ctactacggc 601 aaactcctgc agcccaagta cctgcagccc c //