LOCUS       CK334356                 664 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3019C08-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3019C08 5', mRNA sequence.
ACCESSION   CK334356
VERSION     CK334356.1
DBLINK      BioSample: LIBEST_008622
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     Contact: Dawood B. Dudekula
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            Plate: H3019  row: C  column: 08
            Seq primer: M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3019C08-5"
                     /db_xref="taxon:10090"
                     /clone="H3019C08"
                     /sex="Clones arrayed from a variety of cDNA libraries"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /clone_lib="LIBEST_008622 NIA Mouse 15K cDNA Clone Set"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo  cDNA libraries (including preimplantation stage
                     embryos from  unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos,  extraembryonic part of E7.5
                     embryos, and E12.5 female mesonephros/gonad)  and one
                     newborn ovary cDNA library. Average insert size 1.5 kb.
                     All  source libraries are cloned unidirectionally with
                     Oligo(dT)-Not primers.  References include: (1)
                     Genome-wide expression profiling of mid-gestation
                     placenta and embryo using a 15,000 mouse developmental
                     cDNA microarray,  2000, Proc. Natl. Acad. Sci. U S A, 97:
                     9127-9132; (2) Large-scale cDNA  analysis reveals phased
                     gene expression patterns during preimplantation  mouse
                     develolpment, 2000, Development, 127: 1737-1749; (3)
                     Genome-wide  mapping of unselected transcripts from
                     extraembryonic tissue of 7.5-day  mouse embryos reveals
                     enrichment in the t-complex and under-representation  on
                     the X chromosome, 1998, Hum Mol Genet 7: 1967-1978."
BASE COUNT          181 a          144 c          153 g          186 t
ORIGIN      
        1 cgcaaaggct attggccctc atgatgtatt ggctacactt ttaaacaacc tcaaagttca
       61 ggaaaggcag aacagggttt gtacgactgt agccatagcc attgttgctg aaacatgctc
      121 ccccttcaca gtactgcctg ccttaatgaa tgaatacaga gttcctgaac ttaatgtgca
      181 aaatggagtg ttaaaaacac tctccttctt gtttgagtat atcggcgaaa tggggaaaga
      241 ctacatctat gctgtgacgc ctttacttga ggatgcgtta atggacaggg accttgttca
      301 cagacagaca gcgagtgcag tggtgcagca catgtcactt ggtgtttacg gatttggctg
      361 tgaggattct ctgaatcact tactgaacta tgtgtggcct aatgtgtttg agacatctcc
      421 ccatgtaatc caggcagtta tgggagctct ggagggcctg agggttgcta ttggaccatg
      481 cagaatgttg cagtattgct tacagggtct gttccaccca gctcggaaag tcagagacgt
      541 atattggaaa atttacaact ccatctacat tggctcccag gatgctctca tagcacatta
      601 cccacgaatc tacaatgacg ataagaacac ctatattcgt tacgaacttg actatatctt
      661 gtaa
//