LOCUS CK334356 664 bp mRNA linear EST 12-MAY-2010
DEFINITION H3019C08-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3019C08 5', mRNA sequence.
ACCESSION CK334356
VERSION CK334356.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 664)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT Contact: Dawood B. Dudekula
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
Plate: H3019 row: C column: 08
Seq primer: M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..664
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3019C08-5"
/db_xref="taxon:10090"
/clone="H3019C08"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 181 a 144 c 153 g 186 t
ORIGIN
1 cgcaaaggct attggccctc atgatgtatt ggctacactt ttaaacaacc tcaaagttca
61 ggaaaggcag aacagggttt gtacgactgt agccatagcc attgttgctg aaacatgctc
121 ccccttcaca gtactgcctg ccttaatgaa tgaatacaga gttcctgaac ttaatgtgca
181 aaatggagtg ttaaaaacac tctccttctt gtttgagtat atcggcgaaa tggggaaaga
241 ctacatctat gctgtgacgc ctttacttga ggatgcgtta atggacaggg accttgttca
301 cagacagaca gcgagtgcag tggtgcagca catgtcactt ggtgtttacg gatttggctg
361 tgaggattct ctgaatcact tactgaacta tgtgtggcct aatgtgtttg agacatctcc
421 ccatgtaatc caggcagtta tgggagctct ggagggcctg agggttgcta ttggaccatg
481 cagaatgttg cagtattgct tacagggtct gttccaccca gctcggaaag tcagagacgt
541 atattggaaa atttacaact ccatctacat tggctcccag gatgctctca tagcacatta
601 cccacgaatc tacaatgacg ataagaacac ctatattcgt tacgaacttg actatatctt
661 gtaa
//