LOCUS CK299632 471 bp mRNA linear EST 27-JAN-2011 DEFINITION UI-E-EJ1-ajw-i-16-0-UI.s1 UI-E-EJ1 Homo sapiens cDNA clone UI-E-EJ1-ajw-i-16-0-UI 3', mRNA sequence. ACCESSION CK299632 VERSION CK299632.1 DBLINK BioSample: SAMN00170184 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 471) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT Contact: Soares, MB Cancer Biology & Epigenomics Program Children's Memorial Research Center 2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA Tel: 773 755 6551 Fax: 773 755 6378 Email: mbsoares@childrensmemorial.org Tissue Procurement: Dr. Greg Hageman cDNA Library preparation: Dr. M. Bento Soares, University of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, University of Iowa DNA Sequencing by: Dr. M. Bento Soares, University of Iowa Clone Distribution: Distribution information can be found at http://genome.uiowa.edu/distribution/eye.html Seq primer: M13 FORWARD POLYA=Yes. FEATURES Location/Qualifiers source 1..471 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="UI-E-EJ1-ajw-i-16-0-UI" /tissue_type="fetal eyes, lens, eye anterior segment, optic nerve, retina, Retina Foveal and Macular, RPE and Choroid" /clone_lib="SAMN00170184 UI-E-EJ1" /dev_stage="fetal and adult" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-EJ1 is a subtracted cDNA library constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tags for this library are: fetal eyes, AGAATCAAGA; lens, CGATTAGCGA; eye anterior segment, AATGCCGCAT; optic nerve, CCATTAAGTG; retina, CCGCG; Retina Foveal and Macular, GTCC; RPE and Choroid, ACCTA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI). TAG_LIB=UI-E-EJ1; TAG_SEQ=CCGCG; TAG_TISSUE=human retina" BASE COUNT 95 a 140 c 111 g 125 t ORIGIN 1 tttttttttt tttttcttct tcttaatggc cccagaagtt gacagctttg cccgctcttg 61 gatcaccaag ctccgatagt gttcgtcaca tggatggtcc cacatagact gcccgttggc 121 gaagttgaaa tagtaaatgt cacctgtgat gtcctggcat ggtttccact ctccaggcag 181 tggggccacg atgccctctc gcgccagcca catcagttct ggttccttga tgggatcaat 241 accaatctcc cgggcaaatt caagaatttc ttgctcacta ggaatgtagg tctcatcata 301 atcttcttcc agaaccagct gatctcctat gcggaggggt cgtccagcca tgactcatct 361 gggctcaaac acccgccaga cccccagccc gggcccggtg gcccgctcag gagccggcga 421 gatttcaaac ggaggaactg tcgcctggca gtccgatttt cacctcgtgc c //