LOCUS CK299628 639 bp mRNA linear EST 27-JAN-2011 DEFINITION UI-E-EJ1-ajw-i-06-0-UI.s1 UI-E-EJ1 Homo sapiens cDNA clone UI-E-EJ1-ajw-i-06-0-UI 3', mRNA sequence. ACCESSION CK299628 VERSION CK299628.1 DBLINK BioSample: SAMN00170184 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 639) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT Contact: Soares, MB Cancer Biology & Epigenomics Program Children's Memorial Research Center 2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA Tel: 773 755 6551 Fax: 773 755 6378 Email: mbsoares@childrensmemorial.org Tissue Procurement: Dr. Greg Hageman cDNA Library preparation: Dr. M. Bento Soares, University of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, University of Iowa DNA Sequencing by: Dr. M. Bento Soares, University of Iowa Clone Distribution: Distribution information can be found at http://genome.uiowa.edu/distribution/eye.html Seq primer: M13 FORWARD POLYA=Yes. FEATURES Location/Qualifiers source 1..639 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="UI-E-EJ1-ajw-i-06-0-UI" /tissue_type="fetal eyes, lens, eye anterior segment, optic nerve, retina, Retina Foveal and Macular, RPE and Choroid" /clone_lib="SAMN00170184 UI-E-EJ1" /dev_stage="fetal and adult" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-EJ1 is a subtracted cDNA library constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tags for this library are: fetal eyes, AGAATCAAGA; lens, CGATTAGCGA; eye anterior segment, AATGCCGCAT; optic nerve, CCATTAAGTG; retina, CCGCG; Retina Foveal and Macular, GTCC; RPE and Choroid, ACCTA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI). TAG_LIB=UI-E-EJ1; TAG_SEQ=AGAATCAAGA; TAG_TISSUE=human fetal eyes" BASE COUNT 117 a 166 c 152 g 204 t ORIGIN 1 tttttttttt ttttttttcc tcaaagatct actggtttgt tttggaaata agcacaaggc 61 tgcagctggg ctggtgctca gaggctcagg gccagcactg tgcacagacc ttcggttctg 121 tttggtcagg cctggctgta tggctggtga gcatcttaca tatcacactc ctgtctgcag 181 tgcttcccat cttggggcag aggtggctgg ggtagggcag taggggagac acgtttgggg 241 gctccctgtg gtgcccacgc tgctggcagt gggccactca catctccagg accatgacga 301 ttttggcctt ttcttcaaag gcatccacac actggaccag cttagggtgg tggaggcagt 361 tcatgatgct aatctcctgc cggatattct ctttctcttt tgctgaatat gccttgaaga 421 acttccctgc ccagactttt cgagttttct tttctacaag tcgaaagacc tgtccaaatt 481 tcccagatcc taatctctcc tcaatgtcgt agaagtcaga tactttttgt tcagtattga 541 ttgtcactgt ccggtaatca acctcgggct ccttctcatc atcgtctgac cctccacttc 601 atccttcggc tcttcaggtt tctctcctac cgttgtgag //