LOCUS CK299627 521 bp mRNA linear EST 27-JAN-2011 DEFINITION UI-E-EJ1-ajw-i-04-0-UI.s1 UI-E-EJ1 Homo sapiens cDNA clone UI-E-EJ1-ajw-i-04-0-UI 3', mRNA sequence. ACCESSION CK299627 VERSION CK299627.1 DBLINK BioSample: SAMN00170184 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 521) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT Contact: Soares, MB Cancer Biology & Epigenomics Program Children's Memorial Research Center 2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA Tel: 773 755 6551 Fax: 773 755 6378 Email: mbsoares@childrensmemorial.org Tissue Procurement: Dr. Greg Hageman cDNA Library preparation: Dr. M. Bento Soares, University of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, University of Iowa DNA Sequencing by: Dr. M. Bento Soares, University of Iowa Clone Distribution: Distribution information can be found at http://genome.uiowa.edu/distribution/eye.html Seq primer: M13 FORWARD POLYA=Yes. FEATURES Location/Qualifiers source 1..521 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="UI-E-EJ1-ajw-i-04-0-UI" /tissue_type="fetal eyes, lens, eye anterior segment, optic nerve, retina, Retina Foveal and Macular, RPE and Choroid" /clone_lib="SAMN00170184 UI-E-EJ1" /dev_stage="fetal and adult" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-EJ1 is a subtracted cDNA library constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tags for this library are: fetal eyes, AGAATCAAGA; lens, CGATTAGCGA; eye anterior segment, AATGCCGCAT; optic nerve, CCATTAAGTG; retina, CCGCG; Retina Foveal and Macular, GTCC; RPE and Choroid, ACCTA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI). TAG_LIB=UI-E-EJ1; TAG_SEQ=ACCTA; TAG_TISSUE=RPE and Choroid" BASE COUNT 118 a 132 c 141 g 128 t ORIGIN 1 tttttttttt ttttttttca acacaagtga tctttaattg ctttgacaga acaagggtgt 61 aaacagcgtt agtctacagg cctacccttt ccctttcctc ctctgcagct gaggaaatgg 121 gcagtcccat cgctagaacc ctgttcttag aaattacctg gtctcttgct ctgacagatg 181 ctgagctaat gctgggtcac atatttaaca gctagcctaa agccataggc tggaacagag 241 ttggcaagag cctggctcag gtgtagcgag gagtanagag ggaagacaaa gggagtatca 301 gacccctcac acgggcctct cctccccaaa gatggttcca cctgggtgtg ttcaggagtc 361 actaccttca cgctccctcc tcccccaccc cagctcttcc agggtatacc taaggggtcc 421 agagcaagga agggggcttg agctgggccc gaggaggtga ggttggctgg aatttagggt 481 ggctggcana ggaaagctgg ggaggctgca gccctcgtgc c //