LOCUS       CK299627                 521 bp    mRNA    linear   EST 27-JAN-2011
DEFINITION  UI-E-EJ1-ajw-i-04-0-UI.s1 UI-E-EJ1 Homo sapiens cDNA clone
            UI-E-EJ1-ajw-i-04-0-UI 3', mRNA sequence.
ACCESSION   CK299627
VERSION     CK299627.1
DBLINK      BioSample: SAMN00170184
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     Contact: Soares, MB
            Cancer Biology & Epigenomics Program
            Children's Memorial Research Center
            2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
            Tel: 773 755 6551
            Fax: 773 755 6378
            Email: mbsoares@childrensmemorial.org
            Tissue Procurement: Dr. Greg Hageman
             cDNA Library preparation: Dr. M. Bento Soares, University of Iowa
             cDNA Library Arrayed by: Dr. M. Bento Soares, University of Iowa
             DNA Sequencing by: Dr. M. Bento Soares, University of Iowa
             Clone Distribution: Distribution information can be found at
            http://genome.uiowa.edu/distribution/eye.html
            
            Seq primer: M13 FORWARD
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="UI-E-EJ1-ajw-i-04-0-UI"
                     /tissue_type="fetal eyes, lens, eye anterior segment,
                     optic nerve, retina, Retina Foveal and Macular, RPE and
                     Choroid"
                     /clone_lib="SAMN00170184 UI-E-EJ1"
                     /dev_stage="fetal and adult"
                     /lab_host="DH10B (Life Technologies) (T1 phage resistant)"
                     /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
                     modified polylinker; Site_1: EcoR I; Site_2: Not I;
                     UI-E-EJ1 is a subtracted cDNA library constructed
                     according to Bonaldo, Lennon and Soares, Genome Research,
                     6:791-806, 1996. First strand cDNA synthesis was primed
                     with an oligo-dT primer containing a Not I site. Double
                     stranded cDNA was ligated to an EcoR I adaptor, digested
                     with Not I, and cloned directionally into pT7T3-Pac
                     vector. The oligonucleotide used to prime the synthesis of
                     first-strand cDNA contains a library tag sequence that is
                     located between the Not I site and the (dT)18 tail. The
                     sequence tags for this library are: fetal eyes,
                     AGAATCAAGA; lens, CGATTAGCGA; eye anterior segment,
                     AATGCCGCAT; optic nerve, CCATTAAGTG; retina, CCGCG; Retina
                     Foveal and Macular, GTCC; RPE and Choroid, ACCTA. This
                     library was created for the program, Gene Discovery in the
                     Visual System, supported by National Eye Institute (NEI).
                     TAG_LIB=UI-E-EJ1;
                     TAG_SEQ=ACCTA;
                     TAG_TISSUE=RPE and Choroid"
BASE COUNT          118 a          132 c          141 g          128 t
ORIGIN      
        1 tttttttttt ttttttttca acacaagtga tctttaattg ctttgacaga acaagggtgt
       61 aaacagcgtt agtctacagg cctacccttt ccctttcctc ctctgcagct gaggaaatgg
      121 gcagtcccat cgctagaacc ctgttcttag aaattacctg gtctcttgct ctgacagatg
      181 ctgagctaat gctgggtcac atatttaaca gctagcctaa agccataggc tggaacagag
      241 ttggcaagag cctggctcag gtgtagcgag gagtanagag ggaagacaaa gggagtatca
      301 gacccctcac acgggcctct cctccccaaa gatggttcca cctgggtgtg ttcaggagtc
      361 actaccttca cgctccctcc tcccccaccc cagctcttcc agggtatacc taaggggtcc
      421 agagcaagga agggggcttg agctgggccc gaggaggtga ggttggctgg aatttagggt
      481 ggctggcana ggaaagctgg ggaggctgca gccctcgtgc c
//