LOCUS CK299625 451 bp mRNA linear EST 27-JAN-2011 DEFINITION UI-E-EJ1-ajw-g-20-0-UI.s1 UI-E-EJ1 Homo sapiens cDNA clone UI-E-EJ1-ajw-g-20-0-UI 3', mRNA sequence. ACCESSION CK299625 VERSION CK299625.1 DBLINK BioSample: SAMN00170184 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 451) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT Contact: Soares, MB Cancer Biology & Epigenomics Program Children's Memorial Research Center 2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA Tel: 773 755 6551 Fax: 773 755 6378 Email: mbsoares@childrensmemorial.org Tissue Procurement: Dr. Greg Hageman cDNA Library preparation: Dr. M. Bento Soares, University of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, University of Iowa DNA Sequencing by: Dr. M. Bento Soares, University of Iowa Clone Distribution: Distribution information can be found at http://genome.uiowa.edu/distribution/eye.html Seq primer: M13 FORWARD POLYA=Yes. FEATURES Location/Qualifiers source 1..451 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="UI-E-EJ1-ajw-g-20-0-UI" /tissue_type="fetal eyes, lens, eye anterior segment, optic nerve, retina, Retina Foveal and Macular, RPE and Choroid" /clone_lib="SAMN00170184 UI-E-EJ1" /dev_stage="fetal and adult" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-E-EJ1 is a subtracted cDNA library constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tags for this library are: fetal eyes, AGAATCAAGA; lens, CGATTAGCGA; eye anterior segment, AATGCCGCAT; optic nerve, CCATTAAGTG; retina, CCGCG; Retina Foveal and Macular, GTCC; RPE and Choroid, ACCTA. This library was created for the program, Gene Discovery in the Visual System, supported by National Eye Institute (NEI). TAG_LIB=UI-E-EJ1; TAG_SEQ=GTCC; TAG_TISSUE=Foveal and Macular Retina" BASE COUNT 120 a 111 c 76 g 144 t ORIGIN 1 tttttttttt ttttttttgg tggtttctta aatgatttat tatcgtattc tacatgccaa 61 ctgctttaca tttcccagca gcaagatata ggttctttgg gcatgttctg acatgtctgt 121 ctgagtgaac ttacaccaag taacattggc cctcaggtca aatttctaca attagtcttc 181 caacacccat tttttataat gtcacgtact cttcaagttc ctagaaaaca ccccccaacc 241 ccccccaaaa tttacatatt taattactga attgggttat ccaacctgaa tccaagaaga 301 agccacagct ctcatgtggt ctgcctcagg ctttgggcag ctgagacagc ccatgactgt 361 tgtgtcccat ctgtaagaag tagaaactca ctggtcagtc accaccattg aagaatactg 421 tagctctaca acagcaaatg ggcctcgtgc c //