LOCUS CK299617 719 bp mRNA linear EST 27-JAN-2011
DEFINITION UI-E-EJ1-ajw-g-04-0-UI.s1 UI-E-EJ1 Homo sapiens cDNA clone
UI-E-EJ1-ajw-g-04-0-UI 3', mRNA sequence.
ACCESSION CK299617
VERSION CK299617.1
DBLINK BioSample: SAMN00170184
KEYWORDS EST.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 719)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
PUBMED 8889548
COMMENT Contact: Soares, MB
Cancer Biology & Epigenomics Program
Children's Memorial Research Center
2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
Tel: 773 755 6551
Fax: 773 755 6378
Email: mbsoares@childrensmemorial.org
Tissue Procurement: Dr. Greg Hageman
cDNA Library preparation: Dr. M. Bento Soares, University of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, University of Iowa
DNA Sequencing by: Dr. M. Bento Soares, University of Iowa
Clone Distribution: Distribution information can be found at
http://genome.uiowa.edu/distribution/eye.html
The following repetitive elements were found in this cDNA
sequence: 102-236, >L186_5 242-347, >L1#LINE/L1 502-673,
>MLT1D#LTR/MaLR (matched compliment)
Seq primer: M13 FORWARD
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..719
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="UI-E-EJ1-ajw-g-04-0-UI"
/tissue_type="fetal eyes, lens, eye anterior segment,
optic nerve, retina, Retina Foveal and Macular, RPE and
Choroid"
/clone_lib="SAMN00170184 UI-E-EJ1"
/dev_stage="fetal and adult"
/lab_host="DH10B (Life Technologies) (T1 phage resistant)"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-EJ1 is a subtracted cDNA library constructed
according to Bonaldo, Lennon and Soares, Genome Research,
6:791-806, 1996. First strand cDNA synthesis was primed
with an oligo-dT primer containing a Not I site. Double
stranded cDNA was ligated to an EcoR I adaptor, digested
with Not I, and cloned directionally into pT7T3-Pac
vector. The oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tags for this library are: fetal eyes,
AGAATCAAGA; lens, CGATTAGCGA; eye anterior segment,
AATGCCGCAT; optic nerve, CCATTAAGTG; retina, CCGCG; Retina
Foveal and Macular, GTCC; RPE and Choroid, ACCTA. This
library was created for the program, Gene Discovery in the
Visual System, supported by National Eye Institute (NEI).
TAG_LIB=UI-E-EJ1;
TAG_SEQ=CCGCG;
TAG_TISSUE=human retina"
BASE COUNT 209 a 149 c 128 g 233 t
ORIGIN
1 tttttttttt ttttttttgc atgccaagaa caaggggagt ttattccaga tatgccaaag
61 ttggcttagt actcaaacac tgatcaatag agtttccagt ctggcatgta gaagcttgga
121 agttgcaact ctatcctaaa aacaggtaaa actctgaaca aactaaaaaa tcaacaactc
181 ttcttggatc cctcagaggt cacaggacaa actgttactc tccaaattga agagactgtt
241 gagaaacttg aagctttccc accaggatta ggaacaaaat aaggctgtct cctttcttca
301 tttcctttca acatcatagt ggaagtccca actacacagc actcaggaca ttttggatgt
361 tgctggacat tcattccata tcctggaaag cagcgattaa tccaaaccat ttggagagta
421 ccttcaatat agtatatttg aggtaattgt tctgcacgtc tgccaagaat ttctacttgt
481 tgattaaaaa atctctctat gagattttta gccttatggc tctggagatc agaaatccaa
541 aatgagtctt actaagcaaa aatcaagctg tattcattct gaaagcttta gggggaatcc
601 aattctttgt catttctagc tgtagtggct gccttcatta tttggcatgt ggcttcttct
661 ttgattttca aagaaatcac tgcaagttct ccttttgtca gcgcattcct cctcgtgcc
//