LOCUS CK299615 708 bp mRNA linear EST 27-JAN-2011
DEFINITION UI-E-EJ1-ajw-e-24-0-UI.s1 UI-E-EJ1 Homo sapiens cDNA clone
UI-E-EJ1-ajw-e-24-0-UI 3', mRNA sequence.
ACCESSION CK299615
VERSION CK299615.1
DBLINK BioSample: SAMN00170184
KEYWORDS EST.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 708)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
PUBMED 8889548
COMMENT Contact: Soares, MB
Cancer Biology & Epigenomics Program
Children's Memorial Research Center
2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
Tel: 773 755 6551
Fax: 773 755 6378
Email: mbsoares@childrensmemorial.org
Tissue Procurement: Dr. Greg Hageman
cDNA Library preparation: Dr. M. Bento Soares, University of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, University of Iowa
DNA Sequencing by: Dr. M. Bento Soares, University of Iowa
Clone Distribution: Distribution information can be found at
http://genome.uiowa.edu/distribution/eye.html
The following repetitive elements were found in this cDNA
sequence: 598-708, >ALU
Seq primer: M13 FORWARD
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..708
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="UI-E-EJ1-ajw-e-24-0-UI"
/tissue_type="fetal eyes, lens, eye anterior segment,
optic nerve, retina, Retina Foveal and Macular, RPE and
Choroid"
/clone_lib="SAMN00170184 UI-E-EJ1"
/dev_stage="fetal and adult"
/lab_host="DH10B (Life Technologies) (T1 phage resistant)"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-EJ1 is a subtracted cDNA library constructed
according to Bonaldo, Lennon and Soares, Genome Research,
6:791-806, 1996. First strand cDNA synthesis was primed
with an oligo-dT primer containing a Not I site. Double
stranded cDNA was ligated to an EcoR I adaptor, digested
with Not I, and cloned directionally into pT7T3-Pac
vector. The oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tags for this library are: fetal eyes,
AGAATCAAGA; lens, CGATTAGCGA; eye anterior segment,
AATGCCGCAT; optic nerve, CCATTAAGTG; retina, CCGCG; Retina
Foveal and Macular, GTCC; RPE and Choroid, ACCTA. This
library was created for the program, Gene Discovery in the
Visual System, supported by National Eye Institute (NEI).
TAG_LIB=UI-E-EJ1;
TAG_SEQ=CCGCG;
TAG_TISSUE=human retina"
BASE COUNT 247 a 99 c 133 g 227 t
ORIGIN
1 tttttttttt ttttttcata tattgtaacc cagatgaaac gtttaatatt ttgttaaagt
61 gaaaactata cggtgtatat aaataagggt gttgtttgga gataaaattg tgtgacatga
121 aaatgtacat aagacaatag caggtataaa ctaggttctc tataaacatg aaaaaagggt
181 atccctgtga gtctttagtt cagtcaatgt actgggatgg tacgttagaa atactggaaa
241 actattcttt aggcgaaaat aaatccaatt gtcatgtggt gactattttc cacactctgt
301 tatccacatc tgatagaaca ttaagaaaca gaaaacaaaa aaaagaccca atatagatta
361 caattttaaa ataaaaagta ctgtgtcaag ttcccttcag aatgcccata aagtgaattt
421 gaaattacat gatatttaga aattattgat ttaagcatat tttgatgcaa agaaagatta
481 tgtcaaagtt gtttgtggtt aagtcatttc aagtgtggaa cggaataacg aatataagca
541 aaaataatgc agtttatctc tattttttaa aaattcacat ggtttacatc agtagttgac
601 tgggcacagt agttcactcc tgtaatccca gcaattttgg aggcccaggc aggaggattg
661 cttgaggtca ngagttggag acagcctgng ccgagactcc atctttac
//