LOCUS CK169480 925 bp mRNA linear EST 09-JAN-2011
DEFINITION FGAS044111 Triticum aestivum FGAS: TaLt6 Triticum aestivum cDNA,
mRNA sequence.
ACCESSION CK169480
VERSION CK169480.1
DBLINK BioSample: SAMN00174065
KEYWORDS EST.
SOURCE Triticum aestivum (bread wheat)
ORGANISM Triticum aestivum
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP
clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
REFERENCE 1 (bases 1 to 925)
AUTHORS Allard,F., Crosby,W.L., Danyluk,J., Eudes,F., Frick,M., Gaudet,D.,
Genswein,B., Graf,R., Gulick,P., Hrycan,L.D., Laroche,A.,
Links,M.G., McCarthy,E.L., Monroy,A., Muzak,I., Nilson,D.,
Penniket,C., Roach,J.L. and Sarhan,F.
TITLE Functional Genomics of Abiotic Stress In Wheat and Canola Crops
JOURNAL Unpublished
COMMENT Contact: Wm L Crosby
Bioinformatics
University of Saskatchewan, Department of Computer Science
1C101 Engineering Building, 57 Campus Drive, Saskatoon,
Saskatchewan, S7N 5A9, Canada
Tel: 306 966 1769
Fax: 306 966 2033
Email: fgas_ests@cs.usask.ca
This sequence is the direct result of the Base calling software
Phred (default parameters). It is the raw base calls. To aid in the
identification of the high quality insert the software Lucy
(default parameters) has been run on this sequence. Lucy identified
the region [123,569].
Plate: TaLt605 row: N column: 03.
FEATURES Location/Qualifiers
source 1..925
/organism="Triticum aestivum"
/mol_type="mRNA"
/cultivar="Wheat line CI 14106"
/db_xref="taxon:4565"
/clone_lib="SAMN00174065 Triticum aestivum FGAS: TaLt6"
/lab_host="DH5 alpha"
/note="Organ: Crown; Vector: pGEM-T; SSH (suppression
subtractive hybridization) cDNA library from genotype
CI14106 cold hardened at 2 C for 1 day (24 H)(tester) and
subtracted against genotype CI14106 non-hardened (20 C)
(driver). Nitro-pyrole anchored oligo-dT priming and
non-directional cloning."
BASE COUNT 206 a 278 c 278 g 162 t
ORIGIN
1 accagctcgt gagcatgatt acgccacgct atttatgtga cactatagaa tactcaagct
61 atgcatccaa cgcgtcggga gctctcccat atggtcgagc tgcaggcggc cgcactagtg
121 attagcgtgg tcgcggccga ggtccacaca ctccccacag ccaatcctcc ccagcccaag
181 ctcgcagcca tgcctcccaa gctcgacccg tcccagattg tggaggtgta tgtccgcgtc
241 accggcgggg aggtcggcgc tgcgtcgtcc ctggccccca agatcggccc gctcggtctc
301 tccccaaaga agatcggaga agacatcgcc aaggagacgg ccaaggactg gaagggcctc
361 cgcgtcaccg tcaagctcac cgtgcagaat cgccaggcca aggtgtccgt cgtcccctcc
421 gccgcagccc tcgtcatcaa ggcgctcaag gagcccgaga gggaccgcaa gaaggtcaag
481 aacatcaagc acagcggcaa catcagcctc gacgacgtca tcgagatcgc caagatcatg
541 aaggtccgct ccatgggcca ggagatggcc ggcaccgttc aggagatcct tgggacctgc
601 gtcagcgtcg gctgcaccgt cnacgggcga ggaccccagg atctgcaaac cgggatcgac
661 gacggcgagg tgggagatcc ccgcttaaga aggcttgggt ttgggcaggt ggtatgtgag
721 ggtgctgaaa ttagtcctcc taactgcatt cttcctggat gagttgagaa aagggatggg
781 atattttcat tggtcctacc cgggcctgaa aaagaaactt gggcagcgct tagggggtcc
841 attgggagag aaatttttca cgggttcaat gaggtaccct ggggaacaaa aaccctggcg
901 gggacccccc taacccgcgg gcgtg
//