LOCUS       BV079546                 277 bp    DNA     linear   STS 23-JAN-2004
DEFINITION  D17Jcs64 Mouse Chromosome 17 genomic sequence Mus musculus STS
            genomic, sequence tagged site.
ACCESSION   BV079546
VERSION     BV079546.1
KEYWORDS    STS.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Bergstrom,D.E., Bergstrom,R.A., Munroe,R.J., Lee,B.K.,
            Browning,V.L., You,Y., Eicher,E.M. and Schimenti,J.C.
  TITLE     Overlapping deletions spanning the proximal two-thirds of the mouse
            t complex
  JOURNAL   Mamm. Genome 14 (12), 817-829 (2003)
   PUBMED   14724736
COMMENT     
            Contact: John C. Schimenti
            John C. Schimenti
            The Jackson Laboratory
            600 Main Street, Bar Harbor, ME 04690, USA
            Tel: 207-288-6400
            Fax: 207-288-6078
            Email: jcs@jax.org
            Primer A: TCAGTGCAGACTACCATGTGC
            Primer B: GATGTTAAATGTTTTCCCATATTCA
            STS size: 277
            PCR Profile:
                    Presoak:         94 degrees C for 3 minutes
                    Denaturation:    94 degrees C for 30 seconds
                    Annealing:       55 degrees C for 35 seconds
                    Polymerization:  72 degrees C for 30 seconds
                    PCR Cycles:      40
                    Postsoak:        72 degrees C for 7 minutes
                    Thermal Cycler:  MJ Research PTC-100
            Protocol:
                    Template:        25-100 ng
                    Primer:          200 nM
                    dNTPs:           200 uM
                    Taq Polymerase:  0.025 units/ul
                    Total Volume:    25 ul
            
            Buffer:
                    Tris-HCl:        10 mM
                    pH:              8.3
                    KCl:             50 mM
                    MgCl2:           1.5 mM
                    gelatin          0.001% (w/v).
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Mus musculus"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:10090"
                     /clone_lib="Mouse Chromosome 17 genomic sequence"
                     /note="STSs designed from Celera mouse consensus genome
                     sequence from proximal mouse Chromosome 17."
     STS             1..277
     primer_bind     1..21
     primer_bind     complement(253..277)
BASE COUNT           65 a           18 c           76 g          118 t
ORIGIN      
        1 tcagtgcaga ctaccatgtg caagacagga tatctgtatg tatgtgtgtg tgtgtgtgtg
       61 tgtgtgtgta ttttgtgtgt attctgtgtg tatatgtgta tgtgtatatg tatgcttata
      121 tgtgtgtatg tgtatatatg aatgtaacta tgtgaaggtc tatgtgttta tgtggagtct
      181 gtatgagtat gtatgtacat gtgtatatac gtatgtattg tatatgtgta tgtgtgtgta
      241 tgtatgtgtg tatgaatatg ggaaaacatt taacatc
//