LOCUS       BU987930                 663 bp    mRNA    linear   EST 29-JAN-2011
DEFINITION  HF16C20r HF Hordeum vulgare subsp. vulgare cDNA clone HF16C20
            5-PRIME, mRNA sequence.
ACCESSION   BU987930
VERSION     BU987930.1
DBLINK      BioSample: SAMN00170641
KEYWORDS    EST.
SOURCE      Hordeum vulgare subsp. vulgare (domesticated barley)
  ORGANISM  Hordeum vulgare subsp. vulgare
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BOP
            clade; Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Zhang,H., Sreenivasulu,N., Weschke,W., Stein,N., Rudd,S.,
            Radchuk,V., Potokina,E., Scholz,U., Schweizer,P., Zierold,U.,
            Langridge,P., Varshney,R.K., Wobus,U. and Graner,A.
  TITLE     Large-scale analysis of the barley transcriptome based on expressed
            sequence tags
  JOURNAL   Plant J. 40 (2), 276-290 (2004)
   PUBMED   15447653
COMMENT     Contact: Stein Nils
            Institute of Plant Genetics and Crop Plant Research (IPK)
            Corrensstr. 3, 06466, Gatersleben, Germany
            Tel: 039482-5522
            Fax: 039482-5595
            Email: stein@ipk-gatersleben.de
            Via
            http://gabi.rzpd.de/database/cgi-
            bin/GreenCards.pl.cgi?Mode=ShowBioObject&BioObjectId=225408 a
            detailed clone description is available. Via
            http://pgrc.ipk-gatersleben.de/cr-est/external.php?est=HF16C20r a
            detailed EST description is available. EST mapping information: IPK
            Marker Code GBR1303, On Chromosome 2, Map Position 47.2 Title: A
            1000 loci transcript map of the barley genome - new anchoring
            points for inegrative grass genomics. Authors: N.Stein, M.Prasad,
            U.Scholz, T.Thiel, H.Zhang, M.Wolf, R. Kota, R.K. Varshney,
            D.Perovic, I.Grosse, A.Graner.
            Insert Length: 663   Std Error: 0.00
            Plate: 16  row: C  column: 20
            Seq primer: M13rev.
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Hordeum vulgare subsp. vulgare"
                     /mol_type="mRNA"
                     /cultivar="Barke"
                     /sub_species="vulgare"
                     /db_xref="GABI:247874"
                     /db_xref="taxon:112509"
                     /clone="HF16C20"
                     /tissue_type="caryopsis"
                     /clone_lib="SAMN00170641 HF"
                     /dev_stage="developing caryopsis, 16-25 DAF (days after
                     flowering)"
                     /lab_host="XL10-Gold"
                     /note="Vector: pBluescript SK+; Site_1: EcoRI (5'-end of
                     cDNA); Site_2: XhoI (3'-end of cDNA); developing
                     caryopsis, 16-25 DAF(days after flowing) Due to a cloning
                     artefact caused by the kit, in most cases the EcoRI site
                     is NOT present, as well as the EcoRIadapter used for
                     cloning. To excise the insert, restriction sites upstream
                     EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also
                     due to the cloning system used Blue/white selection for
                     recombinats is not 100% reliable.Average insert size is
                     940 bp"
BASE COUNT          170 a          150 c          181 g          162 t
ORIGIN      
        1 caaagcatca gtatgactgg gatgttgcaa ctacattctc tttcctacag aagtttggtc
       61 ttacagggga attcaagata aatgttgagt gcaaccatgc cactctctct ggacatagct
      121 gccatcacga gcttgagact gcacgcatta atgacattct tggaaacatt gatgcaaaca
      181 ctggtgatcc acaagttggt tgggacacgg atgagttcct tacagacatt tcagaagcta
      241 ccttgattat gtcaagtgta gttaagaatg atggacttgc gcctggtggc ttcaactttg
      301 acgccaaatt gcggagggag agtactgatg ttgaggacct gtttattgcc catatctctg
      361 ggatggacac catggcccgc ggcctccgca atgttgccaa gctgattgag gatggttccc
      421 tggacgagct tgtacgcaag cgctaccaga gctttgacac tgagattggt gccatgatcg
      481 aggctgggaa gggcgacttt gaaacgctag aaaagaaggt cttggagtgg ggcgagccaa
      541 ccgttccatc gggcaaacag gaactggctg agatgctatt ccaatccgct ctgtagatgg
      601 cggcccgtga ttctaggaat aaaaaagcaa gagcgcgacc ttggaacgcc cggccgtcct
      661 cgt
//