LOCUS BU987930 663 bp mRNA linear EST 29-JAN-2011
DEFINITION HF16C20r HF Hordeum vulgare subsp. vulgare cDNA clone HF16C20
5-PRIME, mRNA sequence.
ACCESSION BU987930
VERSION BU987930.1
DBLINK BioSample: SAMN00170641
KEYWORDS EST.
SOURCE Hordeum vulgare subsp. vulgare (domesticated barley)
ORGANISM Hordeum vulgare subsp. vulgare
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP
clade; Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
REFERENCE 1 (bases 1 to 663)
AUTHORS Zhang,H., Sreenivasulu,N., Weschke,W., Stein,N., Rudd,S.,
Radchuk,V., Potokina,E., Scholz,U., Schweizer,P., Zierold,U.,
Langridge,P., Varshney,R.K., Wobus,U. and Graner,A.
TITLE Large-scale analysis of the barley transcriptome based on expressed
sequence tags
JOURNAL Plant J. 40 (2), 276-290 (2004)
PUBMED 15447653
COMMENT Contact: Stein Nils
Institute of Plant Genetics and Crop Plant Research (IPK)
Corrensstr. 3, 06466, Gatersleben, Germany
Tel: 039482-5522
Fax: 039482-5595
Email: stein@ipk-gatersleben.de
Via
http://gabi.rzpd.de/database/cgi-
bin/GreenCards.pl.cgi?Mode=ShowBioObject&BioObjectId=225408 a
detailed clone description is available. Via
http://pgrc.ipk-gatersleben.de/cr-est/external.php?est=HF16C20r a
detailed EST description is available. EST mapping information: IPK
Marker Code GBR1303, On Chromosome 2, Map Position 47.2 Title: A
1000 loci transcript map of the barley genome - new anchoring
points for inegrative grass genomics. Authors: N.Stein, M.Prasad,
U.Scholz, T.Thiel, H.Zhang, M.Wolf, R. Kota, R.K. Varshney,
D.Perovic, I.Grosse, A.Graner.
Insert Length: 663 Std Error: 0.00
Plate: 16 row: C column: 20
Seq primer: M13rev.
FEATURES Location/Qualifiers
source 1..663
/organism="Hordeum vulgare subsp. vulgare"
/mol_type="mRNA"
/cultivar="Barke"
/sub_species="vulgare"
/db_xref="GABI:247874"
/db_xref="taxon:112509"
/clone="HF16C20"
/tissue_type="caryopsis"
/clone_lib="SAMN00170641 HF"
/dev_stage="developing caryopsis, 16-25 DAF (days after
flowering)"
/lab_host="XL10-Gold"
/note="Vector: pBluescript SK+; Site_1: EcoRI (5'-end of
cDNA); Site_2: XhoI (3'-end of cDNA); developing
caryopsis, 16-25 DAF(days after flowing) Due to a cloning
artefact caused by the kit, in most cases the EcoRI site
is NOT present, as well as the EcoRIadapter used for
cloning. To excise the insert, restriction sites upstream
EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also
due to the cloning system used Blue/white selection for
recombinats is not 100% reliable.Average insert size is
940 bp"
BASE COUNT 170 a 150 c 181 g 162 t
ORIGIN
1 caaagcatca gtatgactgg gatgttgcaa ctacattctc tttcctacag aagtttggtc
61 ttacagggga attcaagata aatgttgagt gcaaccatgc cactctctct ggacatagct
121 gccatcacga gcttgagact gcacgcatta atgacattct tggaaacatt gatgcaaaca
181 ctggtgatcc acaagttggt tgggacacgg atgagttcct tacagacatt tcagaagcta
241 ccttgattat gtcaagtgta gttaagaatg atggacttgc gcctggtggc ttcaactttg
301 acgccaaatt gcggagggag agtactgatg ttgaggacct gtttattgcc catatctctg
361 ggatggacac catggcccgc ggcctccgca atgttgccaa gctgattgag gatggttccc
421 tggacgagct tgtacgcaag cgctaccaga gctttgacac tgagattggt gccatgatcg
481 aggctgggaa gggcgacttt gaaacgctag aaaagaaggt cttggagtgg ggcgagccaa
541 ccgttccatc gggcaaacag gaactggctg agatgctatt ccaatccgct ctgtagatgg
601 cggcccgtga ttctaggaat aaaaaagcaa gagcgcgacc ttggaacgcc cggccgtcct
661 cgt
//