LOCUS BR001494 4542 bp DNA linear BCT 16-MAR-2019
DEFINITION TPA_exp: Bifidobacterium longum subsp. longum JCM 1217 BL1,6Gal
gene for exo-beta-1,6-galactobiohydrolase, complete cds.
ACCESSION BR001494
VERSION BR001494.1
KEYWORDS Third Party Data; TPA; TPA:experimental.
SOURCE Bifidobacterium longum subsp. longum
ORGANISM Bifidobacterium longum subsp. longum
Bacteria; Actinomycetota; Actinomycetes; Bifidobacteriales;
Bifidobacteriaceae; Bifidobacterium.
REFERENCE 1 (bases 1 to 4542)
AUTHORS Fujita,K.
TITLE Direct Submission
JOURNAL Submitted (14-FEB-2018) to the DDBJ/EMBL/GenBank databases.
Contact:Kiyotaka Fujita
Kagoshima University, Department of Food Science and
Biotechnology, Laboratory of Applied Carbohydrate Chemistry; 21-24
Korimoto 1, Kagoshima, Kagoshima 890-0065, Japan
URL :http://www.agri.kagoshima-u.ac.jp
REFERENCE 2
AUTHORS Fujita,K., Sakamoto,A., Kaneko,S., Kotake,T., Tsumuraya,Y. and
Kitahara,K.
TITLE Degradative enzymes for type II arabinogalactan side chains in
Bifidobacterium longum subsp. longum
JOURNAL Appl. Microbiol. Biotechnol. 103, 1299-1310 (2019)
REMARK DOI:10.1007/s00253-018-9566-4
COMMENT
PRIMARY TPA_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP
1-4542 AP010888.1 2219758-2224299 c
FEATURES Location/Qualifiers
source 1..4542
/culture_collection="JCM:1217"
/db_xref="taxon:1679"
/mol_type="genomic DNA"
/organism="Bifidobacterium longum subsp. longum"
/strain="JCM 1217"
/sub_species="longum"
CDS 1..4542
/codon_start=1
/EC_number="3.2.1.-"
/experiment="heterologous expression in Escherichia coli"
/gene="BL1,6Gal"
/note="BLLJ_1841"
/product="exo-beta-1,6-galactobiohydrolase"
/protein_id="FAA01256.1"
/transl_table=11
/translation="MRVLSKSLAAMVAAATLVGGGAFAVAGTAYAADNDAITVTPNPW
YANSFDGWGTSLAWFANATGSLGEESAITTNLGDDASKAKAVEYGKQLREQFYQSIFG
DEGLDLNMARYNVGGGNASDVAYGYPFMRQGAAVPGTWKDDATGSGTYGNGVTTKQAD
KDKLAAAFDPTDDNQYDFSKSAAQDWWIERGATGDNPDITDVEAFANSAPWFLTNSGY
ATGGRNSGSNNLANPEKFAQYMAKNVEHLESLGANVDTVEPFNESETSYWGTPGDMAS
KYTDESDDNTKLINNYWDKYYSDKDRSVTPYSNALKKPQEGMHVSNAQQQQTITALAE
ALKDNDDTIIAATDATNSADFVKSYNQYPQAIKDLIGQYNVHAYSDSNQMQSRDIAQA
DGKKLSMSEVDGSWQSGSYNPYGFDNALGMMSKISSNVTRLQSKDFTFWQVVEDLYNM
QMGSNVNPAGENTNWGTVLIDFDCTVAGMDGKLYSERRVNNNGGTTDGLEPCTVIANA
KYNGVKAITHFIHAGDKVIANNDEDNNMTATSDDGKTQTVIHRNSGTSDQTFVIDLSK
YGEIADNAYGELYLTTETSAEDKNAGVDSATPEVFAKTSNVKQAEGSVMIDKAAKTAT
VTVPARSIASIQLTGVTGYAKDAAVETGDTYQLVGKQSGKAVADTTSGDSALSLANVA
SDAENAKKQTWTFTQIEQPTDSERPDLKAYVITNAEGKVLVSKDGTNALSNETVGAAK
SDPAAKWILNTSDGSTYQLLNAATKTNLDVDNSGTTVGTKVGLWQSPSGTSPSANQTW
TLRNVTPTSQKTVNVQTAVNEKAVLPVEVTLYYTWGEGKATVANWDTSKVDVAKEGAY
EATATATDVYGNEFNVTATVYVGALTVSDPVSATVLAGTSASEAKAALEAAPVYLHVK
ASPAFEGDAAKVTWNFDGLDTKLADAKAGDNIAVTGTYQLDDATTIALKGAIYVTAAT
PENVADTASNLTVTNQQTEYSKGDQWKKLTDGDTSAEAWVTWNSAGDYSASPTATIDF
GSECELSSVTITYGDKAPASAKAEYTTDGETWMQFGSDVKPAAGQTVTFKADKGTVNA
TKMRIVNTVNNDYMNATEIQAFVTPVQGAAKNIAAASGTNFSVNFQEGASASKAIDGD
TTSKGWSTWASTASTVDPVATFTFDEAQTITEVKTFFYYDGRASWPKSQTLEYQDEAG
EWHGVGTKDGWKIQAGDAGSGSDGITAADTPTVDFVLGTPVKAKAIRLTNTLQDTKVY
INVAEIQVFAQDSTVLTPQPASDATLGDLRLDGETVEGFDPSKTDYTVDLPVDAEANP
VLQAFATDNAAAVKVTGDAVENGKLGGKAAITVTSADESETKTYTVTFNAFTLASLKV
IGPTKTEYAIGDKLDTAGLKVTAVYQSGDKTKEVPVALDDPQLAIGSFDSTTAGKKAI
TVSYRGVTATFNVTVKANAVAPGPEEQKPGNTNKPGATGNGNKNTVANTGSSVAAIAG
AVALLAAAAGALFMLRKRA"
BASE COUNT 1047 a 1425 c 1286 g 784 t
ORIGIN
1 atgagggtac tgagcaaatc gcttgctgca atggttgcgg cggcaacact agtgggagga
61 ggggcgtttg cggttgccgg cactgcgtat gcggctgata acgatgccat taccgtgacc
121 ccgaacccgt ggtatgccaa cagtttcgat ggctggggca cctcgctggc ttggttcgcc
181 aacgccaccg gcagcctcgg cgaggaatcg gccatcacca ccaatctcgg cgatgacgct
241 tccaaggcta aggctgtgga atacggcaag cagctgcgcg aacagttcta ccagtccatc
301 ttcggtgatg aaggactgga cctgaacatg gcccgctaca acgtgggcgg cggcaatgcc
361 tccgatgttg cctacggcta cccattcatg cgccaaggcg ctgccgtgcc tggcacgtgg
421 aaagatgacg ccaccggctc cggcacgtat ggcaatggcg taaccaccaa gcaggccgac
481 aaagacaagc tggctgcggc attcgacccg actgacgaca accagtatga cttctccaag
541 tccgccgccc aagactggtg gattgagcgc ggtgccaccg gcgataaccc cgacatcacc
601 gatgtagagg ccttcgccaa ctccgctccg tggttcctga ccaacagcgg ttacgccact
661 ggtggacgca actccggtag caataatctt gcaaaccctg agaaattcgc tcagtacatg
721 gccaagaacg tcgagcacct cgaaagcctt ggcgcaaacg ttgacacggt cgagccgttc
781 aacgagtccg agaccagtta ctggggcact ccgggcgaca tggcttcgaa gtacaccgat
841 gagagcgatg acaacaccaa gctcattaac aactactggg ataagtacta ctccgacaaa
901 gataggtccg tcaccccata ctccaacgcg ctgaagaagc cgcaggaggg tatgcatgtc
961 agcaacgccc agcagcagca gacgattacc gcactcgctg aggcgctcaa ggacaatgat
1021 gacaccatca tcgcagccac cgatgccacg aactccgccg acttcgtcaa gtcgtacaac
1081 cagtacccgc aggcgatcaa ggaccttatc ggccagtaca acgttcacgc ctactccgac
1141 agcaaccaga tgcagtcgcg cgatatcgct caggcagacg gcaagaagct gtcgatgagc
1201 gaggtggacg gctcctggca gtccggctcc tacaacccgt acggtttcga caacgcgctg
1261 ggcatgatga gcaagatcag ctccaacgtc acccgcctgc agtccaagga cttcaccttc
1321 tggcaggtgg tcgaggacct ctacaacatg cagatgggct cgaatgtgaa tccggccggt
1381 gagaacacca actggggcac cgtgctcatc gacttcgact gcaccgtggc tggcatggac
1441 ggcaagctct actccgagcg ccgcgtgaac aacaacggcg gtaccaccga tggacttgaa
1501 ccgtgcacgg ttattgcaaa cgccaagtac aacggcgtca aggccatcac ccacttcatc
1561 cacgcgggcg acaaggtcat cgccaacaac gatgaagaca acaacatgac tgccacctcc
1621 gacgatggca agacgcagac cgtcatccac cgcaactccg gcacctctga ccagaccttc
1681 gtcatcgacc tgtcgaagta cggcgagatt gccgacaacg cttacggtga gctctacctg
1741 accaccgaaa cctctgccga agacaagaac gcgggtgtcg attccgccac tccggaagtc
1801 ttcgccaaga ccagcaacgt caagcaagct gaaggctctg tgatgattga caaggctgcc
1861 aagaccgcta cggtcactgt gcccgcccgt tctatcgcct ccatccagct cactggcgtg
1921 accggctacg ctaaggatgc tgccgtcgag accggcgaca cttaccagct cgttggtaag
1981 cagtccggca aggccgtggc tgataccact tctggtgatt ccgcgctgtc cctggccaac
2041 gtcgcttccg atgccgagaa cgccaagaag cagacttgga cctttaccca gatcgagcag
2101 cccaccgact ccgagcgccc tgatctcaag gcttatgtga ttactaacgc cgaaggcaag
2161 gtgctggtgt ccaaggatgg cacgaacgcg ctttccaacg aaacggttgg ggccgctaaa
2221 tccgacccgg ctgccaagtg gattctcaac acttccgatg gttcgaccta ccagctgctc
2281 aatgccgcga ctaagacgaa ccttgatgtg gataactctg gtaccacagt cggcacgaag
2341 gttggcttgt ggcagtcacc gagcggcact tcgccgtccg ccaaccagac atggactctg
2401 cgcaatgtaa cgccgaccag ccagaagacc gtgaacgtgc agaccgccgt taacgagaag
2461 gccgtgcttc cggtcgaagt cacgctctac tacacttggg gcgaaggcaa ggccacggtt
2521 gccaactggg atacttccaa ggtcgatgtg gccaaggaag gcgcctacga agccaccgct
2581 accgccaccg atgtgtacgg caacgagttc aacgtcaccg ctacggtcta cgttggcgcg
2641 ctcaccgttt ccgatccggt atcggctaca gtgctggccg gcaccagtgc gagcgaggcg
2701 aaggccgcgc ttgaggctgc gccggtgtac ctacacgtca aggcatcgcc tgcattcgag
2761 ggcgatgcgg ctaaggttac gtggaacttc gatgggcttg ataccaagct cgccgatgcc
2821 aaggctggcg acaacattgc cgtgaccggt acttaccagc tggacgacgc gaccacgatt
2881 gcactgaagg gcgcgatcta tgtcaccgcc gccacgcctg agaatgtggc cgacactgct
2941 tccaacctga ccgtgaccaa ccagcagacg gaatacagca agggcgatca gtggaagaag
3001 ctcaccgatg gtgacacgtc agctgaagcc tgggtgacgt ggaactctgc tggtgactat
3061 tccgccagcc cgaccgccac gattgacttc ggctctgagt gtgagcttag cagcgtgacc
3121 attacgtatg gtgacaaggc tccggcttcc gccaaggccg agtacaccac tgatggcgag
3181 acgtggatgc aattcggtag cgatgttaag cctgccgcag gccagacggt gacgttcaag
3241 gccgataagg gcacagtgaa tgccacgaag atgcgcattg tgaacaccgt gaacaacgac
3301 tacatgaacg ccaccgaaat tcaggcattc gtgacgccgg ttcagggtgc tgcgaagaac
3361 atcgccgcgg cctctggcac gaacttctcg gtgaacttcc aggagggtgc ctccgcttcc
3421 aaggccatcg atggtgacac tacgtcaaag ggttggtcca cttgggcttc caccgcctcg
3481 acggtggacc cggtcgccac gttcaccttc gacgaagctc agaccatcac cgaagtgaag
3541 accttcttct actacgatgg tcgtgcgtct tggccgaaga gccagacgct ggaataccag
3601 gatgaggctg gcgaatggca tggagtcggt accaaggatg gctggaagat acaggccggc
3661 gatgccggct ctggctccga cggcatcacc gccgccgaca ccccgaccgt tgacttcgtg
3721 ctcggcaccc cggtaaaggc caaggccatc cgcctgacta acacattgca ggacaccaag
3781 gtgtacatca acgtggctga gatccaggtg ttcgcacaag acagcacggt actcaccccg
3841 cagccagcat ccgatgccac gctgggcgac ctgcgtcttg acggcgaaac cgttgaaggc
3901 ttcgacccgt ccaagaccga ctacacggtt gatctgccgg tcgacgccga ggcaaacccg
3961 gtgctgcagg ccttcgccac cgacaatgcc gccgccgtca aggtgactgg cgacgcggtt
4021 gagaacggca agcttggcgg caaggccgcc attacggtga cctcagccga cgagtctgag
4081 acgaagacct acacggtgac cttcaacgcc ttcactttgg cttcgctcaa ggtgatcgga
4141 cccacgaaga ccgagtacgc catcggcgac aagctcgata ccgccggtct gaaggtgact
4201 gccgtctacc agagtggcga caagaccaag gaagtgccgg tcgctcttga cgacccgcag
4261 cttgcgattg gctcgttcga ctccaccacc gcaggcaaga aggcgattac cgtctcctac
4321 cgtggtgtga ccgcgacctt caacgtcacg gtcaaggcca acgcagtcgc ccctggccct
4381 gaagaacaga agcccggcaa caccaacaag cccggtgcca ccggcaacgg caacaagaac
4441 acggtggcca acaccggttc cagtgttgcc gccatcgctg gcgctgtcgc tctgctggcc
4501 gctgccgcgg gtgcactgtt catgctgcgc aagcgtgcat ag
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