LOCUS BQ462765 550 bp mRNA linear EST 29-JAN-2011
DEFINITION HI01O22T HI Hordeum vulgare subsp. vulgare cDNA clone HI01O22
5-PRIME, mRNA sequence.
ACCESSION BQ462765
VERSION BQ462765.1
DBLINK BioSample: SAMN00170639
KEYWORDS EST.
SOURCE Hordeum vulgare subsp. vulgare (domesticated barley)
ORGANISM Hordeum vulgare subsp. vulgare
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP
clade; Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
REFERENCE 1 (bases 1 to 550)
AUTHORS Zhang,H., Sreenivasulu,N., Weschke,W., Stein,N., Rudd,S.,
Radchuk,V., Potokina,E., Scholz,U., Schweizer,P., Zierold,U.,
Langridge,P., Varshney,R.K., Wobus,U. and Graner,A.
TITLE Large-scale analysis of the barley transcriptome based on expressed
sequence tags
JOURNAL Plant J. 40 (2), 276-290 (2004)
PUBMED 15447653
COMMENT Contact: Stein Nils
Institute of Plant Genetics and Crop Plant Research (IPK)
Corrensstr. 3, 06466, Gatersleben, Germany
Tel: 039482-5522
Fax: 039482-5595
Email: stein@ipk-gatersleben.de
Via
http://gabi.rzpd.de/database/cgi-
bin/GreenCards.pl.cgi?Mode=ShowBioObject&BioObjectId=133631 a
detailed clone description is available. Via
http://pgrc.ipk-gatersleben.de/cr-est/external.php?est=HI01O22T a
detailed EST description is available. EST mapping information: IPK
Marker Code GBM1121, On Chromosome 2, Map Position 26.2 Title: A
1000 loci transcript map of the barley genome - new anchoring
points for inegrative grass genomics. Authors: N.Stein, M.Prasad,
U.Scholz, T.Thiel, H.Zhang, M.Wolf, R. Kota, R.K. Varshney,
D.Perovic, I.Grosse, A.Graner.
Insert Length: 550 Std Error: 0.00
Plate: 1 row: O column: 22
Seq primer: T3.
FEATURES Location/Qualifiers
source 1..550
/organism="Hordeum vulgare subsp. vulgare"
/mol_type="mRNA"
/cultivar="Barke"
/sub_species="vulgare"
/db_xref="taxon:112509"
/clone="HI01O22"
/tissue_type="female inflorescences"
/clone_lib="SAMN00170639 HI"
/dev_stage="female inflorescences (approx. 3 mm in size)"
/lab_host="XL10-Gold"
/note="Vector: pBluescript SK+; Site_1: EcoRI (5'-end of
cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning
artefact caused by the kit, in most cases the EcoRI site
is NOT present, as well as the EcoRIadapter used for
cloning. To excise the insert, restriction sites upstream
EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also
due to the cloning system used Blue/white selection for
recombinats is not 100% reliable."
BASE COUNT 87 a 199 c 179 g 85 t
ORIGIN
1 caccaccacc cccatccatc agacaagaga gagagagagc gtctgcagtc tgcagcctgc
61 agcagccatg gccaaggagg tgagcgagga gccggagcac gccgcgccgg cgcgcaagga
121 ctactcggac ccgccgccgg cgccgctgtt cgacatgggg gagctccgga tgtggtcctt
181 ctaccgggcg ctcatcgccg agttcgtcgc cacgctgctc tttctctaca tcaccgtcgc
241 caccgtcatc ggctacaagg tgcagtcggc ggccgacccg tgcgccggcg tcggggtgct
301 cggcatcgcc tgggccttcg gcggcatgat cttcgaccta gtctactgca ccgccggcat
361 ctcgggcgga cacatcaacc cggccgtcac cttcggcctc ctcctcgcgc gcaaggtgtc
421 gctgctgcgc gccgtcatgt acatcgtggc gcagcgcgcc ggcggcatcg tgggcgccgg
481 catcgtgaag ggcatcatga aggacgcgta ccacgccaac ggcggcggcg ccaacatggt
541 cgcctcgggc
//