LOCUS BM489143 594 bp mRNA linear EST 23-FEB-2011 DEFINITION pgm2n.pk010.a20 Normalized Chicken Breast Muscle, Leg Muscle, and Epiphyseal Growth Plate cDNA library (pgm2n) Gallus gallus cDNA clone pgm2n.pk010.a20 5' similar to ref|NP_251369.1 (NC_002516) hypothetical protein [Pseudomonas aeruginosa] pir|A83310 hypothetical protein PA2679 [imported] - Pseudomonas aeruginosa (strain PAO1) gb|AAG06067.1|AE004696_1 (AE004696) hypothetical protein [Pseudomonas aeruginosa], mRNA sequence. ACCESSION BM489143 VERSION BM489143.1 DBLINK BioSample: SAMN00170127 KEYWORDS EST. SOURCE Gallus gallus (chicken) ORGANISM Gallus gallus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda; Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae; Phasianinae; Gallus. REFERENCE 1 (bases 1 to 594) AUTHORS Carre,W., Wang,X., Porter,T.E., Nys,Y., Tang,J., Bernberg,E., Morgan,R., Burnside,J., Aggrey,S.E., Simon,J. and Cogburn,L.A. TITLE Chicken genomics resource: sequencing and annotation of 35,407 ESTs from single and multiple tissue cDNA libraries and CAP3 assembly of a chicken gene index JOURNAL Physiol. Genomics 25 (3), 514-624 (2006) PUBMED 16554550 COMMENT Contact: Larry A. Cogburn University of Delaware Townsend Hall, Newark, DE 19717, USA Tel: 302-831-1335 Fax: 302-831-2822 Email: cogburn@udel.edu, www.chickest.udel.edu. FEATURES Location/Qualifiers source 1..594 /organism="Gallus gallus" /mol_type="mRNA" /strain="Commercial broiler and Ottawa Res. Centre Strains 90 & 21" /db_xref="taxon:9031" /clone="pgm2n.pk010.a20" /sex="male and female" /tissue_type="Breast muscle, leg muscle and epiphyseal growth plate" /clone_lib="SAMN00170127 Normalized Chicken Breast Muscle, Leg Muscle, and Epiphyseal Growth Plate cDNA library (pgm2n)" /dev_stage="Breast,leg:Embryo(d19);post-hatch(1d,1,3,5,7, 9,11 weeks);growth plate(1d,7d,14d post-hatch)" /lab_host="E. coli EMDH10B" /note="Vector: pCMVSPORT6; Library made from equivalent pools of total RNA isolated from each tissue (embryonic muscle 33.3%; juvenile muscle 33.3%; and epiphyseal growth plate 33.3% of the final RNA pool). Single pass sequencing from 5'-end" BASE COUNT 107 a 188 c 184 g 115 t ORIGIN 1 gccccgttct gccccagagc aagcctgtcc ctgctggctg tagtgtgagc ggccttgttc 61 ctctgtcccg acactgcagg agggcctggg agcggacagg ccctgtgctc ccagcatggc 121 cacgcagatg tttgagggca cacagcacgc ggccgtctac ctgaagtacc gctttgcccc 181 tggcaaggag ctgaaggaca tcatcctcgc ctacctgcag gagaaggctc ccagctgcac 241 ccagctggct gtggatgtgg gctgcggatc ggggcaaggc acggccttcc tggcagaccg 301 ctttgcaaag gtggtgggca ccgacatcag ccaggcgcag atccaggagg ccaaggctgc 361 cccctctcct cccaacatct cctacctggt gtgcccagcg gaggagctgc cctttgagga 421 tggctcggtt gatctcctgg cctcgttcac ggccgcgcac tggtttgata ttgggaagtt 481 catgaacgag gtgaagcgtg tgctgcggcc gggcggctgc gtggccatca gcacctacac 541 cattgacatg agcctgcact acagggactg ctctgaaaag ctgacccaaa tctt //