LOCUS       BM489143                 594 bp    mRNA    linear   EST 23-FEB-2011
DEFINITION  pgm2n.pk010.a20 Normalized Chicken Breast Muscle, Leg Muscle, and
            Epiphyseal Growth Plate cDNA library (pgm2n) Gallus gallus cDNA
            clone pgm2n.pk010.a20 5' similar to ref|NP_251369.1 (NC_002516)
            hypothetical protein [Pseudomonas aeruginosa] pir|A83310
            hypothetical protein PA2679 [imported] - Pseudomonas aeruginosa
            (strain PAO1) gb|AAG06067.1|AE004696_1 (AE004696) hypothetical
            protein [Pseudomonas aeruginosa], mRNA sequence.
ACCESSION   BM489143
VERSION     BM489143.1
DBLINK      BioSample: SAMN00170127
KEYWORDS    EST.
SOURCE      Gallus gallus (chicken)
  ORGANISM  Gallus gallus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
            Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes;
            Phasianidae; Phasianinae; Gallus.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Carre,W., Wang,X., Porter,T.E., Nys,Y., Tang,J., Bernberg,E.,
            Morgan,R., Burnside,J., Aggrey,S.E., Simon,J. and Cogburn,L.A.
  TITLE     Chicken genomics resource: sequencing and annotation of 35,407 ESTs
            from single and multiple tissue cDNA libraries and CAP3 assembly of
            a chicken gene index
  JOURNAL   Physiol. Genomics 25 (3), 514-624 (2006)
   PUBMED   16554550
COMMENT     Contact: Larry A. Cogburn
            University of Delaware
            Townsend Hall, Newark, DE 19717, USA
            Tel: 302-831-1335
            Fax: 302-831-2822
            Email: cogburn@udel.edu, www.chickest.udel.edu.
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Gallus gallus"
                     /mol_type="mRNA"
                     /strain="Commercial broiler and Ottawa Res. Centre Strains
                     90 & 21"
                     /db_xref="taxon:9031"
                     /clone="pgm2n.pk010.a20"
                     /sex="male and female"
                     /tissue_type="Breast muscle, leg muscle and epiphyseal
                     growth plate"
                     /clone_lib="SAMN00170127 Normalized Chicken Breast Muscle,
                     Leg Muscle, and Epiphyseal Growth Plate cDNA library
                     (pgm2n)"
                     /dev_stage="Breast,leg:Embryo(d19);post-hatch(1d,1,3,5,7,
                     9,11 weeks);growth plate(1d,7d,14d post-hatch)"
                     /lab_host="E. coli EMDH10B"
                     /note="Vector: pCMVSPORT6; Library made from equivalent
                     pools of total RNA isolated from each tissue (embryonic
                     muscle 33.3%; juvenile muscle 33.3%; and epiphyseal growth
                     plate 33.3% of the final RNA pool). Single pass sequencing
                     from 5'-end"
BASE COUNT          107 a          188 c          184 g          115 t
ORIGIN      
        1 gccccgttct gccccagagc aagcctgtcc ctgctggctg tagtgtgagc ggccttgttc
       61 ctctgtcccg acactgcagg agggcctggg agcggacagg ccctgtgctc ccagcatggc
      121 cacgcagatg tttgagggca cacagcacgc ggccgtctac ctgaagtacc gctttgcccc
      181 tggcaaggag ctgaaggaca tcatcctcgc ctacctgcag gagaaggctc ccagctgcac
      241 ccagctggct gtggatgtgg gctgcggatc ggggcaaggc acggccttcc tggcagaccg
      301 ctttgcaaag gtggtgggca ccgacatcag ccaggcgcag atccaggagg ccaaggctgc
      361 cccctctcct cccaacatct cctacctggt gtgcccagcg gaggagctgc cctttgagga
      421 tggctcggtt gatctcctgg cctcgttcac ggccgcgcac tggtttgata ttgggaagtt
      481 catgaacgag gtgaagcgtg tgctgcggcc gggcggctgc gtggccatca gcacctacac
      541 cattgacatg agcctgcact acagggactg ctctgaaaag ctgacccaaa tctt
//