LOCUS BM173327 475 bp mRNA linear EST 04-DEC-2001 DEFINITION 900681 Avicennia marina leaf cDNA Library Avicennia marina cDNA clone Am900681 5' similar to L-isoaspartyl methyltransferase (L07941) of Triticum aestivum, mRNA sequence. ACCESSION BM173327 VERSION BM173327.1 DBLINK BioSample: SAMN00161299 KEYWORDS EST. SOURCE Avicennia marina ORGANISM Avicennia marina Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Lamiales; Acanthaceae; Avicennioideae; Avicennia. REFERENCE 1 (bases 1 to 475) AUTHORS Parani,M., Mehta,P., Sivaprakash,K.R. and Parida,A. TITLE Expressed sequence tags from the mangrove species Avicennia marina JOURNAL Unpublished COMMENT Contact: Parani M / Parida A Department of Plant Molecular Biology M. S. Swaminathan Research Foundation III Cross Street, Taramani Institutional Area, Chennai 600 113, India Tel: 91-44-2351319 Fax: 91-44-2351319 Email: mangrovegenes@mssrf.res.in BLAST search in BLASTX (Non-redundant)using default parameters as on November 6th 2001 Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..475 /organism="Avicennia marina" /mol_type="mRNA" /strain="Pichavaram" /db_xref="taxon:82927" /clone="Am900681" /sex="hermaphrodite" /tissue_type="Leaf" /clone_lib="SAMN00161299 Avicennia marina leaf cDNA Library" /dev_stage="1 year old plant" /note="A one-year-old plant from the natural mangrove habitat in Pichavaram, Tamil Nadu, India was collected and brought to the M.S.Swaminatan Research Foundation. The whole plant was treated with the nutrient solution supplemented with 500 mM NaCl for 48 hours. Poly(A+)mRNA from the leaf tissue was purified and cDNA was prepared using Superscript Lambda System (Life Technologies, Cat. No. 19643-014). The cDNAs were size fractionated over SizeSep-400 spun column (Amersham-Pharmacia Biotech Cat No. 27-5105-01) and cloned in 5'Sal I - 3' Not I of pSPORT1 (Life Technologies Cat. No. 15383-011). The ESTs were sequenced from the 5' end using M13/pUC18 reverse primer in an Automated Sequencer (ABI310, Applied Biosystems) and submitted after editing to remove the vector and adapter sequences. BLAST search in BLASTX (Non-redundant)was carried out on 7th August, 2000 using default parameters and the results reported under putative identification/comment" BASE COUNT 104 a 132 c 111 g 128 t ORIGIN 1 cgcggttctt cgctctccgt tatcgtgtct ggctggccgt gtccgacgcc tctatacttt 61 tccattttat gcttcactcc tcgccgcttc caccccagcc ttcgcctcat ccgtctgcct 121 caagtggccc gcgcgcttac cgccttctcc tcccgctgct gcagccttcc taatcctcac 181 ctcttcaaag ggaactctct cttctcccgc atggagcgat tctggactgg aagcagtatc 241 aagtaacaac aaaaaattgg tggagcattt gcagctatgg aatgataaga tcaaagaaag 301 tagcagaagt gatggaaaca attgacaggg cattggttgt gcccgagggt gaaccgcata 361 tgttgatagt ccatgcaaac ggcataatgc tactattctg cacctatatg catgccatgt 421 gccttgagtg gtggcgaaca ttgaagcctg gcatgcatgc ttggattagg ttgaa //