LOCUS       BM173327                 475 bp    mRNA    linear   EST 04-DEC-2001
DEFINITION  900681 Avicennia marina leaf cDNA Library Avicennia marina cDNA
            clone Am900681 5' similar to L-isoaspartyl methyltransferase
            (L07941) of Triticum aestivum, mRNA sequence.
ACCESSION   BM173327
VERSION     BM173327.1
DBLINK      BioSample: SAMN00161299
KEYWORDS    EST.
SOURCE      Avicennia marina
  ORGANISM  Avicennia marina
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
            Pentapetalae; asterids; lamiids; Lamiales; Acanthaceae;
            Avicennioideae; Avicennia.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Parani,M., Mehta,P., Sivaprakash,K.R. and Parida,A.
  TITLE     Expressed sequence tags from the mangrove species Avicennia marina
  JOURNAL   Unpublished
COMMENT     Contact: Parani M / Parida A
            Department of Plant Molecular Biology
            M. S. Swaminathan Research Foundation
            III Cross Street, Taramani Institutional Area, Chennai 600 113,
            India
            Tel: 91-44-2351319
            Fax: 91-44-2351319
            Email: mangrovegenes@mssrf.res.in
            BLAST search in BLASTX (Non-redundant)using default parameters as
            on November 6th 2001
            Seq primer: M13 Reverse.
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Avicennia marina"
                     /mol_type="mRNA"
                     /strain="Pichavaram"
                     /db_xref="taxon:82927"
                     /clone="Am900681"
                     /sex="hermaphrodite"
                     /tissue_type="Leaf"
                     /clone_lib="SAMN00161299 Avicennia marina leaf cDNA
                     Library"
                     /dev_stage="1 year old plant"
                     /note="A one-year-old plant from the natural mangrove
                     habitat in Pichavaram, Tamil Nadu, India was collected and
                     brought to the M.S.Swaminatan Research Foundation. The
                     whole plant was treated with the nutrient solution
                     supplemented with 500 mM NaCl for 48 hours. Poly(A+)mRNA
                     from the leaf tissue was purified and cDNA was prepared
                     using Superscript Lambda System (Life Technologies, Cat.
                     No. 19643-014). The cDNAs were size fractionated over
                     SizeSep-400 spun column (Amersham-Pharmacia Biotech Cat
                     No. 27-5105-01) and cloned in 5'Sal I - 3' Not I of
                     pSPORT1 (Life Technologies Cat. No. 15383-011). The ESTs
                     were sequenced from the 5' end using M13/pUC18 reverse
                     primer in an Automated Sequencer (ABI310, Applied
                     Biosystems) and submitted after editing to remove the
                     vector and adapter sequences. BLAST search in BLASTX
                     (Non-redundant)was carried out on 7th August, 2000 using
                     default parameters and the results reported under putative
                     identification/comment"
BASE COUNT          104 a          132 c          111 g          128 t
ORIGIN      
        1 cgcggttctt cgctctccgt tatcgtgtct ggctggccgt gtccgacgcc tctatacttt
       61 tccattttat gcttcactcc tcgccgcttc caccccagcc ttcgcctcat ccgtctgcct
      121 caagtggccc gcgcgcttac cgccttctcc tcccgctgct gcagccttcc taatcctcac
      181 ctcttcaaag ggaactctct cttctcccgc atggagcgat tctggactgg aagcagtatc
      241 aagtaacaac aaaaaattgg tggagcattt gcagctatgg aatgataaga tcaaagaaag
      301 tagcagaagt gatggaaaca attgacaggg cattggttgt gcccgagggt gaaccgcata
      361 tgttgatagt ccatgcaaac ggcataatgc tactattctg cacctatatg catgccatgt
      421 gccttgagtg gtggcgaaca ttgaagcctg gcatgcatgc ttggattagg ttgaa
//