LOCUS       BI751677                 460 bp    mRNA    linear   EST 25-SEP-2001
DEFINITION  Ta01_13d04_R
            Ta01_AAFC_ECORC_Fusarium_graminearum_inoculated_wheat_heads
            Triticum aestivum cDNA clone Ta01_13d04, mRNA sequence.
ACCESSION   BI751677
VERSION     BI751677.1
DBLINK      BioSample: SAMN00164294
KEYWORDS    EST.
SOURCE      Triticum aestivum (bread wheat)
  ORGANISM  Triticum aestivum
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BOP
            clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Ouellet,T., Dan,H., Koul,A., Chapados,J., Couroux,P., De Moors,A.,
            Harris,L.J., Hattori,J.I., Robert,L.S., Singh,J.A., Sprott,D. and
            Tinker,N.A.
  TITLE     Expressed Sequence Tags from Wheat Heads 24 Hours after Spray
            Inoculation with Fusarium graminearum
  JOURNAL   Unpublished
COMMENT     Contact: Ouellet, Therese
            Eastern Cereal and Oilseed Research Centre
            Agriculture and Agri-food Canada
            Neatby Bldg., Central Experimental Farm, Ottawa, Ontario, K1A 0C6,
            CANADA
            Tel: (613) 759-1658
            Fax: (613) 759-1701
            Email: ouellettr@agr.gc.ca.
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Triticum aestivum"
                     /mol_type="mRNA"
                     /cultivar="Frontana"
                     /db_xref="taxon:4565"
                     /clone="Ta01_13d04"
                     /tissue_type="heads"
                     /clone_lib="SAMN00164294
                     Ta01_AAFC_ECORC_Fusarium_graminearum_inoculated_wheat_head
                     s"
                     /dev_stage="anthesis"
                     /note="Vector: pGEM-T easy; Site_1: EcoRI; Site_2: EcoR1;
                     Controled chamber-grown wheat heads were spray inoculated
                     at mid-anthesis with a Fusarium graminearum macroconidial
                     suspension (50,000 spores/ml) and kept under intermitent
                     misting for 24 hours, then collected and immediately
                     frozen in liquid nitrogen."
BASE COUNT          110 a          134 c          104 g          105 t
ORIGIN      
        1 acatacaggt cggtgaagaa gtttgaagag gtctacttcc agagcatcag ccagcctaac
       61 cacgcgcgtc caccgaagac ggcgctggac tagagctcgt gaagaaaacg ctcgarctga
      121 tcgacgctcc agtnagggaa cctggctgac tgaatgcctc caacaacgtg actnahgcgc
      181 catgcatgth gcatgagaha aggctgacca gatcagctcc acaagaacat ctaacctata
      241 acgatagtcc aacatgaatc cctttttatc taacttcatt gacggcttca tcgtcgcccg
      301 trtcggcttt gatccgagcc ctctgagagg ttagcagatg atgcaccctt tgctttagct
      361 cttgcgttcc gatttggctc tgcaccgaca cccggatggc gtcttccggt ccaaactctc
      421 tgtatctcct caattcatcg tcgacgccat catcagcggc
//