LOCUS BG604429 330 bp mRNA linear EST 16-APR-2001 DEFINITION WHE0833_E01_I01ZS Wheat vernalized crown cDNA library Triticum aestivum cDNA clone WHE0833_E01_I01, mRNA sequence. ACCESSION BG604429 VERSION BG604429.1 DBLINK BioSample: SAMN00160091 KEYWORDS EST. SOURCE Triticum aestivum (bread wheat) ORGANISM Triticum aestivum Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum. REFERENCE 1 (bases 1 to 330) AUTHORS Anderson,O.D., Chao,S., Choi,D.W., Close,T.J., Fenton,R.D., Han,P.S., Hsia,C.C., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J., Seaton,C.L. and Tong,J.C. TITLE The structure and function of the expressed portion of the wheat genomes - Vernalized crown cDNA library JOURNAL Unpublished COMMENT Contact: Olin Anderson US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center 800 Buchanan Street, Albany, CA 94710, USA Tel: 5105595773 Fax: 5105595818 Email: oandersn@pw.usda.gov Sequence have been trimmed to remove vector sequence and low quality sequence with phred score less than 20 Seq primer: Stratagene SK primer. FEATURES Location/Qualifiers source 1..330 /organism="Triticum aestivum" /mol_type="mRNA" /cultivar="Chinese Spring" /db_xref="taxon:4565" /clone="WHE0833_E01_I01" /tissue_type="Crown tissue of seedling" /clone_lib="SAMN00160091 Wheat vernalized crown cDNA library" /dev_stage="Five-week old seedling" /lab_host="E. coli SOLR" /note="Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors)." BASE COUNT 71 a 75 c 74 g 110 t ORIGIN 1 ctgcaaggct gtgacatact tcaaaacttg cctgcaaccc ttggtcatct ccaaacactt 61 gagcatctta gggtgtcatg tcgttatgat attggtgagc tagctgactc tctctgcaat 121 cttcatgatc ttcggtttat tgatttgtgg aggggcgctg agcttccaca gttgccttgt 181 tcatttggta atttagtgag gggggaggat ctaaacctat ctagtggctt caacctcaac 241 cagctaccag aatcttttgg caacctttat tttcttaggt ctctgaacat gtcgagctgc 301 tctgagctcg aacaattgcc tggatctttt //