LOCUS BG088225 539 bp mRNA linear EST 12-MAY-2010 DEFINITION H3150C12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3150C12 5', mRNA sequence. ACCESSION BG088225 VERSION BG088225.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 539) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG088225.1. Other_ESTs: H3150C12-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3150 row: C column: 12 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..539 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3150C12-5" /db_xref="taxon:10090" /clone="H3150C12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 132 a 131 c 155 g 121 t ORIGIN 1 cgtgtgggtg tcaaacgctg cacaggctcg ggtcgctccc gcttctcccg cgtcgccggc 61 ggctgcagcc ttgcactcgc gctggccagg aacggagggc atggctgcca gcttctggga 121 cctgaggctt tggaggagga aatgactgtg gagggccatt ttgttccatc tgcatttctg 181 acacggaaac ttcctagggt gcaggcttag gggacaggaa agtttctctt gaagggcttc 241 acatttgtaa caaagaagat aaaaatgaca acttcatcta tcagacggca gatgaaaaac 301 attgtgaaca attactcaga ggctgaaatc aaagtccggg aggccacctc caatgacccg 361 tggggcccat ccagctctct gatgactgag attgctgacc tgacctacaa tgtggtagcg 421 ttctcggaga tcatgagcat ggtttggaag cgacttaatg accatggcaa gaactggcgg 481 catgtataca aggcactgac actgctggac taccttatca agacaggttc tgagcgggt //