LOCUS BG087995 627 bp mRNA linear EST 12-MAY-2010 DEFINITION H3147D02-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3147D02 5', mRNA sequence. ACCESSION BG087995 VERSION BG087995.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 627) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG087995.1. Other_ESTs: H3147D02-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3147 row: D column: 02 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..627 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3147D02-5" /db_xref="taxon:10090" /clone="H3147D02" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 157 a 177 c 170 g 123 t ORIGIN 1 gagctgtgaa catgtgctat cgaaccctct acatcgggac aggagctgac atggatgtgt 61 gccttacaaa ctatggtcac tgtaactacg tgtccgggaa acatgcctgc atattctacg 121 atgagaatac caaacattat gagctgttaa actacagtga gcatgggaca acggtggaca 181 atgtgctgta ttcatgtgac ttctctgaga agaccccgcc aaccccccca agcagtattg 241 ttgccaaagt acagagtgtc atcaggcgcc gccgacacca gaaacaggat gaagagccaa 301 gtgaggaggc agccatgatg agttcccagg cccaggggcc acagcggaga ccctgcaatt 361 gcaaagccag cagctcaagc ttgattgggg gcagtggggc cggctgggag ggcacagcct 421 tactccacca tggcagctac atcaagctgg gctgcctgca gtttgtcttc agcatcactg 481 agtttgcgac caaacagccc aaaggcgatg ccagcctgct gcaggatggg gtcttggctg 541 agaaactctc tctcaagccc catcagggcc ctgtgctgcg ctccaactcc gttccctagg 601 ccattggcct ggacgcccac ccaagac //