LOCUS       BG087521                 592 bp    mRNA    linear   EST 26-JAN-2001
DEFINITION  H3140F12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3140F12 5', mRNA sequence.
VERSION     BG087521.1
DBLINK      BioSample: SAMN00163148
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
            Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
  TITLE     Verification and initial annotation of NIA mouse 15K cDNA clone set
  JOURNAL   Unpublished
COMMENT     Other_ESTs: H3140F12-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            This clone set has been freely distributed to the community. Please
            visit for details.
            Plate: H3140  row: F  column: 12
            Seq primer: -21M13 Reverse
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Mus musculus"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          210 a          162 c           51 g          169 t
        1 aataattacc ctatttatct tatttcaact aaaagtctca tcacaaacat tcccactggc
       61 accttcacca aaatcactaa caaccataaa agtaaaaacc ccttgagaat taaaatgaac
      121 gaaaatctat ttgcctcatt cattacccca acaataatag gattcccaat cgttgtagcc
      181 atcattatat ttccttcaat cctattccca tcctcaaaac gcctaatcaa caaccgtctc
      241 cattctttcc aacactgact agttaaactt attatcaaac aaataatgct aatccacaca
      301 ccaaaaggac gaacatgaac cctaataatt gtttccctaa tcatatttat tggatcaaca
      361 aatctcctag gccttttacc acatacattt acacctacta cccaactatc cataaatcta
      421 agtatagcca ttccactatg agctggagcc gtaattacag gcttccgaca caaactaaaa
      481 agctcacttg cccacttcct tccacaagga actccaattt cactaattcc aatacttatt
      541 attattgaaa caattagcct atttattcaa ccaatggcat tagcagtccg gc