LOCUS       BG087490                 512 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3140D04-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3140D04 5', mRNA sequence.
ACCESSION   BG087490
VERSION     BG087490.2
DBLINK      BioSample: LIBEST_008622
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced gi:12570054.
            Other_ESTs: H3140D04-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3140  row: D  column: 04
            Seq primer: -21M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3140D04-5"
                     /db_xref="taxon:10090"
                     /clone="H3140D04"
                     /sex="Clones arrayed from a variety of cDNA libraries"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /clone_lib="LIBEST_008622 NIA Mouse 15K cDNA Clone Set"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo  cDNA libraries (including preimplantation stage
                     embryos from  unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos,  extraembryonic part of E7.5
                     embryos, and E12.5 female mesonephros/gonad)  and one
                     newborn ovary cDNA library. Average insert size 1.5 kb.
                     All  source libraries are cloned unidirectionally with
                     Oligo(dT)-Not primers.  References include: (1)
                     Genome-wide expression profiling of mid-gestation
                     placenta and embryo using a 15,000 mouse developmental
                     cDNA microarray,  2000, Proc. Natl. Acad. Sci. U S A, 97:
                     9127-9132; (2) Large-scale cDNA  analysis reveals phased
                     gene expression patterns during preimplantation  mouse
                     develolpment, 2000, Development, 127: 1737-1749; (3)
                     Genome-wide  mapping of unselected transcripts from
                     extraembryonic tissue of 7.5-day  mouse embryos reveals
                     enrichment in the t-complex and under-representation  on
                     the X chromosome, 1998, Hum Mol Genet 7: 1967-1978."
BASE COUNT          125 a          126 c          141 g          120 t
ORIGIN      
        1 actggagata tttattccaa ttctaacttg aagaataaaa atgacctctt tgaatacaaa
       61 tttgcaactg gccagtggac ggagtggaag attgaaggga ggttgcctgt tgccaggtca
      121 gcccatgggg ctacagtgta cagtgataag ctctggatct ttgctggtta tgatggcaat
      181 gccaggttga acgacatgtg gacaattggc ctccaagacc gagagctcac atgctgggag
      241 gaggtggccc agagtggtga gatcccccca tcctgttgca acttccctgt ggctgtgtgc
      301 cgggataaga tgttcgtgtt ctcaggacag agtggagcca agataactaa caacctcttc
      361 cagtttgaat tcaaagacaa gacgtggaca cgcatcccta ctgagcacct gcttcggggc
      421 tccccacccc cgccacagcg gcgctacgga cgcaccatgg tggcctttga ccgccatctc
      481 tatgtgtttg ggggtgcagc ggacaataca ct
//