LOCUS BG087149 635 bp mRNA linear EST 12-MAY-2010 DEFINITION H3136B04-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3136B04 5', mRNA sequence. ACCESSION BG087149 VERSION BG087149.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 635) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG087149.1. Other_ESTs: H3136B04-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3136 row: B column: 04 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..635 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3136B04-5" /db_xref="taxon:10090" /clone="H3136B04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 161 a 156 c 180 g 138 t ORIGIN 1 cctagatctt gtctccgtgg cagcagccgg ttgcagttgc cggagagaag cttctaccga 61 cagtcgtcag ggccgcagag cgctgccgag gcggctcgtc agctaagctg tgctgctcca 121 gggcaggcac catgttgcat tcatctttac gcccagcatc tagcagtgtc ggcatgtagt 181 aggcactcaa gaaatgtatg ttgaatgaac gatgtctgtg acaagcaact ggactttcct 241 ctttcctgac ccttgctcct atgacatacc tcctgattgc cactgtcacc ccttcaaagc 301 agacctctag ggcttcctgg aagggaaaca gctatggcca aggacatctt gggcgaggca 361 gggctgcatt ttgatgaact gaacaagctc cgagtgttgg acccagaggt cacccagcag 421 actgtggagc tcaaggagga gtgcaaggac tttgtggaca aaattggcca gtttcagaaa 481 attgttggtg gtctaattga gcttgttgat cagcttgcca aagaagcaga gaacgagaag 541 atgaaggcca ttggtgctcg gaacttgctg aagtccatag cgaagcagag agaagcccag 601 cagcagcagc tgcaggccct gatagcagaa aagaa //