LOCUS BG086774 616 bp mRNA linear EST 12-MAY-2010 DEFINITION H3130D06-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3130D06 5', mRNA sequence. ACCESSION BG086774 VERSION BG086774.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 616) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG086774.1. Other_ESTs: H3130D06-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3130 row: D column: 06 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..616 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3130D06-5" /db_xref="taxon:10090" /clone="H3130D06" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 100 a 218 c 156 g 142 t ORIGIN 1 ggacaatcct cctcgtcctc ccggcccccg ctcgccaatg ctgtggcctg cacctgcatc 61 gctctctggg aacctcggag acctcaactc cttcgtactg gtcttacccc ttcgtactgg 121 tcggggttta ctgcaggtcc acctccccta ggccatggcc aggtggggga aggtcactcc 181 aggacgctag tgactctgcc cagctctgct ccgcctctct gcttatgcac ctgccgcccc 241 ctcgcactct ctgccactgc ggttgcagtt ccggcccgtc cctcttctgc aggccagccc 301 agcgggcagc ctcggttcct gactcctggt ctgtgcttgt gggcggcctg aaccacctca 361 atccggacaa ttgccaaacc caccctgggc tgtagctggg aaccggttgt ggctcctgcc 421 agtctagacc ccggggaaca atttatgcac gtgacccagc cacctaagac tctcctggcc 481 caggtctgtg gctggtattc ccattggctt actgttctgt ctcactgggg gcacccctac 541 cctatactgt agaattcttg gaatgacaga gcacttggga agacccagct cacatgtcaa 601 gatgtcgcag agcact //