LOCUS       BG086535                 679 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3127D12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3127D12 5', mRNA sequence.
VERSION     BG086535.2
DBLINK      BioSample: SAMN00163148
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced BG086535.1.
            Other_ESTs: H3127D12-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            This clone set has been freely distributed to the community. Please
            visit for details.
            Plate: H3127  row: D  column: 12
            Seq primer: -21M13 Reverse
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Mus musculus"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          184 a          199 c          147 g          149 t
        1 actgcaccta caagcaaacc agcatcagct ggtggagaac ttctcgactt gctaggagac
       61 atcaccctga caggtgctcc agctgctgct cctacccctg cctcagtgcc acagatatcc
      121 cagcccccct tcttgttgga tgggctttcc tcacagcctc tcttcaatga catcgctcca
      181 ggcatcccct ccatcacagc gtacagtaag aacggcttga agatagagtt cacctttgaa
      241 cggtcaaaca ccaaccccag cgtaacagtg ataacgatac aggcctctaa cagcacagag
      301 ctagacatga cggactttgt tttccaggct gcagtaccaa agacattcca gctgcagctt
      361 ctgtctccta gcagcagcgt tgtcccagcc tttaatacag ggaccatcac acaagtcatt
      421 aaagttctga atccacagaa gcaacagctg cgaatgcgga tcaagctcac atataatcac
      481 aagggctcgg caatgcaaga tctagcagaa gtgaacaatt tcccccctca gtcctggcaa
      541 tgaggatgca gcaccattct cattcttatc ccactcaatc aaaggaattc tgggaaggag
      601 gttgtgattg ctggcaagtc cccccaactg taccatgggc acgaggagct gagagaactg
      661 ttgaggaggg ttttcctca