LOCUS       BG086534                 728 bp    mRNA    linear   EST 26-JAN-2001
DEFINITION  H3127D10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3127D10 5', mRNA sequence.
ACCESSION   BG086534
VERSION     BG086534.1
DBLINK      BioSample: LIBEST_008622
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
            Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
  TITLE     Verification and initial annotation of NIA mouse 15K cDNA clone set
  JOURNAL   Unpublished (2001)
COMMENT     Other_ESTs: H3127D10-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3127  row: D  column: 10
            Seq primer: -21M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3127D10-5"
                     /db_xref="taxon:10090"
                     /clone="H3127D10"
                     /sex="Clones arrayed from a variety of cDNA libraries"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /clone_lib="LIBEST_008622 NIA Mouse 15K cDNA Clone Set"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo  cDNA libraries (including preimplantation stage
                     embryos from  unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos,  extraembryonic part of E7.5
                     embryos, and E12.5 female mesonephros/gonad)  and one
                     newborn ovary cDNA library. Average insert size 1.5 kb.
                     All  source libraries are cloned unidirectionally with
                     Oligo(dT)-Not primers.  References include: (1)
                     Genome-wide expression profiling of mid-gestation
                     placenta and embryo using a 15,000 mouse developmental
                     cDNA microarray,  2000, Proc. Natl. Acad. Sci. U S A, 97:
                     9127-9132; (2) Large-scale cDNA  analysis reveals phased
                     gene expression patterns during preimplantation  mouse
                     develolpment, 2000, Development, 127: 1737-1749; (3)
                     Genome-wide  mapping of unselected transcripts from
                     extraembryonic tissue of 7.5-day  mouse embryos reveals
                     enrichment in the t-complex and under-representation  on
                     the X chromosome, 1998, Hum Mol Genet 7: 1967-1978."
BASE COUNT          182 a          167 c          184 g          191 t
ORIGIN      
        1 tccctctccc tctccctctc cctctccctc tccctctccc tctacctcta cctctacctc
       61 tacctctacc tctacctctc cctctctcct ctccatgtag ctctggctgt tcaaaactcc
      121 ctctacagac caggctggtc ttgaactcag agatccacct gcctctgcct cctgagtgct
      181 gggattaagg gaatgttcca ccagctctca tactctctca aagtatttct aaggaattga
      241 cattgaacag aggtcagaat aaagggagag agtaaactgt atgattgttg aaactgttct
      301 gtggagagta ggtgcaaagg ttctaggtga gactgtgctt ggcgagaccg aagatggaga
      361 agatagtgag gctgaggctg gtagcctggc agagatagga gggatggtga aatattcagg
      421 aggtagagtg cgaggagtct tacaggctgg ggataagatc tggggttctt ttaaatgtaa
      481 tcaagtgact ttggagatct gctttcatct ctaagttatt cagcttctga gatgaaaagt
      541 agctaatatg ctattagcag gatggtccag accagacaca cattaaggct gtagtggagg
      601 tggttaaagt ggtggggtca tctggatgtg gcagctaccg ctagcagtcc agagccttgg
      661 aggccagagc tgnctcttnt gaagctaaca ggagccctgt cttaaaaaaa aaaaaaaagg
      721 tcggncgn
//