LOCUS       BG085699                 644 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3116E02-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3116E02 5', mRNA sequence.
VERSION     BG085699.2
DBLINK      BioSample: SAMN00163148
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced BG085699.1.
            Other_ESTs: H3116E02-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            This clone set has been freely distributed to the community. Please
            visit for details.
            Plate: H3116  row: E  column: 02
            Seq primer: -21M13 Reverse
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Mus musculus"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          132 a          169 c          158 g          185 t
        1 gccctctaca cagtcggcta cagcacctcc ctcgtcaccc tcaccactgc catggtcatc
       61 ttgtgccgct tccggaagct gcactgtacc cgtaacttca tccacatgaa cctgtttgta
      121 tccttcatgc tgagagctat ctctgtcttc atcaaagact ggatcttgta tgccgagcag
      181 gacagcagtc attgcttcgt ttccaccgtg gaatgcaaag ctgtcatggt tttctttcac
      241 tactgcgtgg tgtccaacta cttctggctg ttcattgaag gcctatacct ctttacactg
      301 ctggtggaga ccttcttccc tgagaggaga tatttctact ggtatgccat cattggctgg
      361 gggacaccta ctgtgtgtgt aactgtgtgg gctgtgctga ggctctactt tgatgatgcg
      421 ggatgctggg atatgaatga cagcacagct ctgtggtggg tgatcaaagg ccctgtagtt
      481 ggctctataa tggttaactt tgtgcttttc atcggcatca tcatcatcct tgtgcagaag
      541 ctgcagtccc cagacatggg aggcaatgag tcgagcatct acttctgcgt gcagaaatgc
      601 tactgcaagc cacagcgggc tcagcagcac tcttgcaaga tgtc