LOCUS BG085651 635 bp mRNA linear EST 12-MAY-2010 DEFINITION H3115H07-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3115H07 5', mRNA sequence. ACCESSION BG085651 VERSION BG085651.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 635) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG085651.1. Other_ESTs: H3115H07-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3115 row: H column: 07 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..635 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3115H07-5" /db_xref="taxon:10090" /clone="H3115H07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 143 a 157 c 221 g 114 t ORIGIN 1 tagtgggtga gggcatcttc aagatgccca cagtgacagt gccccagcta gagctggatg 61 tggggctggg ccatgaagcc caggctggtg aagcagccaa gagtgagggt gggataaagt 121 tgaagttgcc cacactgggg accggaagca gaggagaggg cgttgagccc cagggccccg 181 aggcccagcg caccttccac ctctcattgc ccgatgtgga actcacgtca ccagtgagta 241 gccacgctga gtaccaggta gttgagggtg atggggatgg tgggcacaaa ctcaaggttc 301 ggctgcccct gtttggtctg gcaaaggcca aggaagggat agaagttgga gaaaaggcta 361 agagtccaaa gctcaggcta ccccgagtgg gcttcagcca gagtgagtcg gtctccggag 421 aaggctctcc aagtcctgag gaggaggaag aaggcagtgg ggaaggggct tccagtcgcc 481 ggggtagggt gagggtccgc ctgcctcggg taggcttggc ttccccttct aaagtctcta 541 agggacagga gggtgatgca acctccaagt ccccagttgg ggagaagtca cccaaattcc 601 gttttcctag ggtgtcctta agccccaggc ccgga //