LOCUS       BG085572                 609 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3114H06-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3114H06 5', mRNA sequence.
ACCESSION   BG085572
VERSION     BG085572.2
DBLINK      BioSample: LIBEST_008622
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced gi:12568136.
            Other_ESTs: H3114H06-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3114  row: H  column: 06
            Seq primer: -21M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3114H06-5"
                     /db_xref="taxon:10090"
                     /clone="H3114H06"
                     /sex="Clones arrayed from a variety of cDNA libraries"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /clone_lib="LIBEST_008622 NIA Mouse 15K cDNA Clone Set"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo  cDNA libraries (including preimplantation stage
                     embryos from  unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos,  extraembryonic part of E7.5
                     embryos, and E12.5 female mesonephros/gonad)  and one
                     newborn ovary cDNA library. Average insert size 1.5 kb.
                     All  source libraries are cloned unidirectionally with
                     Oligo(dT)-Not primers.  References include: (1)
                     Genome-wide expression profiling of mid-gestation
                     placenta and embryo using a 15,000 mouse developmental
                     cDNA microarray,  2000, Proc. Natl. Acad. Sci. U S A, 97:
                     9127-9132; (2) Large-scale cDNA  analysis reveals phased
                     gene expression patterns during preimplantation  mouse
                     develolpment, 2000, Development, 127: 1737-1749; (3)
                     Genome-wide  mapping of unselected transcripts from
                     extraembryonic tissue of 7.5-day  mouse embryos reveals
                     enrichment in the t-complex and under-representation  on
                     the X chromosome, 1998, Hum Mol Genet 7: 1967-1978."
BASE COUNT          179 a          111 c          119 g          200 t
ORIGIN      
        1 gatgtttttg tttttaaatt ggattctttt ccagggtcag caacaacaaa aaactcctga
       61 aaaataagca ctactccaga aaagtagtat tctttccccc tgtcacggca agcctagaaa
      121 gcctttgagg ataaaatctt tataggctgg tcataattaa aaattctctt aggggcagca
      181 tttctttgct ggattccttt tgggattggc cttaggaaga agaataggtg gcttttctgt
      241 tgcttactgt atgtgagaaa agtggtcaat tgtagaaaac agtgaccttt cctaaaatgg
      301 caagatccat atttctattt agctcctaac agcacactgc ttaagacagt ttaagaagtg
      361 gctttccaat tatggtacat ggacctttta aaatcattat ggtgattgaa acccctattc
      421 taggcaagca gtctgccact gagtaatgta cccagcccca agtgaatatt tgaaattgaa
      481 aatttgagca aggaaagtat atattcatgt gcttttatag gcctgacttt tgttgttctt
      541 ttagacagtc ttagtccagg ctggccttaa actttgtttg cccaagataa acttgaactg
      601 atcttcatg
//